Photo-protective and Anti-melanogenic Effect from Phenolic Compound of Olive Leaf (Olea europaea L. var. Kalamata) Extracts on the Immortalized Human Keratinocytes and B16F1 Melanoma Cells

被引：0

作者：

Ha, Ju Yeon ^{[1
,2
]}

Choi, Hyun Kyung ^{[3
]}

Oh, Myoung Jin ^{[3
]}

Choi, Hae-Yeon ^{[1
,2
]}

Park, Chang Seo ^{[3
]}

Shin, Han-Seung ^{[1
,2
]}

机构：

[1] Dongguk Univ, Dept Food Sci & Technol, Seoul 100715, South Korea

[2] Dongguk Univ, Inst Lotus Funct Food Ingredients, Seoul 100715, South Korea

[3] Dongguk Univ, Dept Chem & Biochem Engn, Seoul 100715, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2009年 / 18卷 / 05期

关键词：

olive leaf extract; oleuropein; phenolic compound; photo-protective; anti-melanogenic effect; ANTIOXIDANT ACTIVITY; SKIN; INHIBITION; OLEUROPEIN; ACID;

D O I：

暂无

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ethylacetate and butanol fractions of leaf extracts (OLE) showed the higher contents of total phenolic compounds than hexane and water fractions. Oleuropein contents were 4.21 +/- 0.57, 3.92 +/- 0.43, 0.32 +/- 0.03, 5.76 +/- 0.32, and 32.47 +/- 0.25 mg/100 g for ethanol extract, and hexane, chloroform, ethyl acetate, and butanol fraction, respectively. Treatment of ultraviolet-B (UVB) irradiated cells with 3 OLEs prepared by using ethylacetate and butanol at concentrations 0.001, 0.005, and 0.01% respectively showed significant recover), of cell viabilities. Treatment of dexametason 1 mM reduced tumor necrotic factor (TNF)-alpha secretion by about 40%. UVB irradiated immortalized human keratinocytes (HaCaT) cells were treated with 3 different OLEs at the same concentrations. Ethylacetate fraction showed the strongest inhibition activity with respect of reduction of the elevated TNF-alpha. Cytotoxicity of OLEs on the B16-F1 cells was evaluated through thiazolyl blue tetrazolium bromide (MTT) assay. Ethylacetate fraction has no cytotoxicity in the range of 0.005-0.01%. A slight cytotoxicity was observed at the concentration of 0.1% butanol fraction of OLE that caused 10% decrease in cell viability.

引用

页码：1193 / 1198

页数：6