Express primer tool for high-throughput gene cloning and expression

被引:26
|
作者
Yoon, JR [1 ]
Laible, PD [1 ]
Gu, M [1 ]
Scott, HN [1 ]
Collart, FR [1 ]
机构
[1] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA
关键词
D O I
10.2144/02336bc03
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers. specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount Of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.
引用
收藏
页码:1328 / +
页数:5
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