Express primer tool for high-throughput gene cloning and expression

被引：26

作者：

Yoon, JR ^{[1
]}

Laible, PD ^{[1
]}

Gu, M ^{[1
]}

Scott, HN ^{[1
]}

Collart, FR ^{[1
]}

机构：

[1] Argonne Natl Lab, Biosci Div, Argonne, IL 60439 USA

来源：

BIOTECHNIQUES | 2002年 / 33卷 / 06期

关键词：

D O I：

10.2144/02336bc03

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-throughput approaches for gene cloning and expression require the development of new nonstandard tools for molecular biologists and biochemists. We introduce a Web-based tool to design primers. specifically for the generation of expression clones for both laboratory-scale and high-throughput projects. The application is designed not only to allow the user complete flexibility to specify primer design parameters but also to minimize the amount Of manual intervention needed to generate a large number of primers for the simultaneous amplification of multiple target genes.

引用

页码：1328 / +

页数：5

共 50 条

[1] HTP-OligoDesigner: An Online Primer Design Tool for High-Throughput Gene Cloning and Site-Directed Mutagenesis
Camilo, Cesar M.
Lima, Gustavo M. A.
Maluf, Fernando V.
Guido, Rafael V. C.
Polikarpov, Igor
[J]. JOURNAL OF COMPUTATIONAL BIOLOGY, 2016, 23 (01) : 27 - 29
[2] High-throughput cloning and expression in recalcitrant bacteria
Geertsma E.R.
Poolman B.
[J]. Nature Methods, 2007, 4 (9) : 705 - 707
[3] High-throughput cloning and expression in recalcitrant bacteria
Geertsma, Eric R.
Poolman, Bert
[J]. NATURE METHODS, 2007, 4 (09) : 705 - 707
[4] A Versatile and Efficient High-Throughput Cloning Tool for Structural Biology
Geertsma, Eric R.
Dutzler, Raimund
[J]. BIOCHEMISTRY, 2011, 50 (15) : 3272 - 3278
[5] High throughput methods for gene cloning and expression
Dieckman, L
Gu, MY
Stols, L
Donnelly, MI
Collart, FR
[J]. PROTEIN EXPRESSION AND PURIFICATION, 2002, 25 (01) : 1 - 7
[6] High-Throughput Gene-Expression Profiling
Dyer, Randy
Glazer, Celeste
[J]. GENETIC ENGINEERING & BIOTECHNOLOGY NEWS, 2011, 31 (13): : 38 - 39
[7] High-throughput imaging of brain gene expression
Brown, VM
Ossadtchi, A
Khan, AH
Cherry, SR
Leahy, RM
Smith, DJ
[J]. GENOME RESEARCH, 2002, 12 (02) : 244 - 254
[8] PCR primer selection tool optimized for high-throughput proteomics and structural genomics
Canaves, JM
Morse, A
West, B
[J]. BIOTECHNIQUES, 2004, 36 (06) : 1040 - 1042
[9] High-throughput cloning and expression library creation for functional proteomics
Festa, Fernanda
Steel, Jason
Bian, Xiaofang
Labaer, Joshua
[J]. PROTEOMICS, 2013, 13 (09) : 1381 - 1399
[10] QuantPrime - a flexible tool for reliable high-throughput primer design for quantitative PCR
Arvidsson, Samuel
Kwasniewski, Miroslaw
Riano-Pachon, Diego Mauricio
Mueller-Roeber, Bernd
[J]. BMC BIOINFORMATICS, 2008, 9 (1)

← 1 2 3 4 5 →