A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin

被引：26
|
作者：
Chen, ZL [1 ]
Iding, K
Lütkemeyer, D
Lehmann, J
机构：
[1] Inst Biotechnol, Dept Cell Engn, Beijing 100071, Peoples R China
[2] Univ Bielefeld, Inst Cell Culture Technol, D-33501 Bielefeld, Germany
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 10期
关键词：
protein-free medium; prothrombin; recombinant CHO cell;
D O I：
10.1023/A:1005665530028
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A chemically defined protein free medium, DF6S, was developed for the cultivation of a recombinant Chinese hamster ovary cell line (CHO2DS) producing human prothrombin in suspension batch culture. DF6S was formulated by optimizing DME/F12 with amino acids and supplementing the optimized DME/F12 with aurintricarboxylic acid, ethanolamine, ferric sulfate, Pluronic F68, putrescine and sodium pyruvate. From a seeding density of 2.3 x 10(5) cells ml(-1), CHO2DS cells grown in suspension in DF6S medium reached a maximal cell density of 1.92 x 10(6) cells ml(-1) with an accumulated prothrombin concentration of 16.7 mg l(-1) after 6 days in culture.
引用
收藏
页码：837 / 841
页数：5
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