Motilimonas eburnea gen. nov., sp nov., isolated from coastal sediment

A novel Gram-stain-negative, non-spore-forming, facultatively anaerobic bacterium, designated YH6(T), was isolated from marine sediment in Weihai, China. Cells of starin YH6(T) were motile, straight rods that formed ivory-white colonies on 2216E agar. Optimal growth occurred at 28-33 degrees C (range 15-37 degrees C), in the presence of 2-4% (w/v) NaCl (range 1-8 %) and at pH 7.5-8.5 (range pH 6.5-9.0). The sole respiratory lipoquinone was Q-8, and the major fatty acids (> 10 %) were C-16:0 and summed feature 3 (C-16 :1 omega 7c/iso-C-15:0 2-OH). The polar lipids profile of the novel strain consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and several other unknown lipids (phospholipids, lipid and phosphoaminolipid). The G+C content of the genomic DNA was 46.5 mol%. The closest type strain phylogenetically to strain YH6(T) was Vibrio variabilis (92.99% 16S rRNA gene sequence similarity) followed by Paramoritella alkaliphila (92.55 %), Pseudoalteromonas aurantia (92.20 %) and Pseudoalteromonas citrea (92.20 %). Phylogenetic analysis of the 16S rRNA gene sequence placed the novel strain in the order Alteromonadales, class Gammaproteobacteria. On the basis of the 16S rRNA gene sequence data as well as physiological and biochemical characteristics, we concluded that strain YH6(T) represents a novel species of a new genus. We propose the name of Motilimonas eburnea gen. nov., sp. nov. for this novel species. The type strain of the novel species is YH6(T) (=KCTC 42594(T) = MCCC 1H00122(T)).