Type 2 diabetes mellitus and diabetic nephropathy with Angiotensin II type 1 receptor gene variants: A case control study in a Turkish population

Objective: This study was performed to determine the polymorphism frequencies of the Angiotensin II type 1 receptor (AT1) A1166C, and to examine the role of this polymorphism in type-2 diabetes (T2DM) and diabetic nephropathy (DN) development. Design: This is a research study. Setting: Clinic for Internal Medicine, Artvin State Hospital, Turkey Subjects: The study consisted of 259 (135 T2DM and DN + 124 controls) persons referred to the Clinic for Internal Medicine, Artvin State Hospital. Intervention: This study used peripheral blood samples. Main outcome measures: Two hundred and fifty-nine genomic DNAs were analyzed. AT1 gene A1166C polymorphisms were determined using polymerase chain reaction (PCR), restriction fragment length polymorphism and electrophoresis. PCR products were digested with restriction enzyme HindIII and were analyzed in 2% agarose gel electrophoresis. Results: The frequencies of A1166C genotypes were found to be 32.3% AC and 67.7% CC in controls and 73% AC and 27% CC in total patient group. Statistically, it was found that the AT1 gene A1166C polymorphism genotype was significantly different between the control group and the total patient's group. Conclusions: As a result, we can say that there is an interaction between the AT1 gene A1166C polymorphism with T2DM and DN.