Conversion of D-carnitine into L-carnitine with stereospecific carnitine dehydrogenases

被引：9
|
作者：
Hanschmann, H [1 ]
Kleber, HP [1 ]
机构：
[1] UNIV LEIPZIG,FAK BIOWISSENSCH PHARM & PSYCHOL,INST BIOCHEM,D-04103 LEIPZIG,GERMANY
来源：
BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 07期
关键词：
D O I：
10.1023/A:1018399001074
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
D-Carnitine was converted to L-carnitine by resting and permeabilized cells as well as with purified stereospecific carnitine dehydrogenases from Agrobacterium sp. With permeabilized cells only 11% of D-carnitine was converted into L-carnitine. Using highly stereospecific D- and L-carnitine dehydrogenases from Agrobacterium sp. (pH 8.5, 50 mM D-carnitine, 1 mM NAD(+), 0.1 mM NADH, 25-fold excess of L-carnitine dehydrogenase) almost 50% of the D-carnitine could be converted into L-carnitine.
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页码：679 / 682
页数：4
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