OCCURRENCE OF CITRUS HUANGLONGBING IN CUBA AND ASSOCIATION OF THE DISEASE WITH CANDIDATUS LIBERIBACTER ASIATICUS

被引：1

作者：

Luis, M. ^{[1
]}

Collazo, C. ^{[1
]}

Llauger, R. ^{[1
]}

Blanco, E. ^{[2
]}

Pena, I. ^{[1
]}

Lopez, D. ^{[1
]}

Gonzalez, C. ^{[1
]}

Casin, J. C. ^{[2
]}

Batista, L. ^{[1
]}

Kitajima, E. ^{[6
]}

Tanaka, F. A. O. ^{[6
]}

Salaroli, R. B. ^{[6
]}

Teixeita, D. C. ^{[3
]}

Martins, E. C. ^{[3
]}

Bove, J. M. ^{[4
,5
]}

机构：

[1] Res Inst Trop Fruit Crops, Havana, Cuba

[2] Natl Ctr Plant Hlth La Habana, Havana, Cuba

[3] Fundecitrus, Araraquara, SP, Brazil

[4] Univ Bordeaux 2, F-33076 Bordeaux, France

[5] Inst Natl Rech Agron, Bordeaux, France

[6] Escola Super Agr Luis de Queiroz, Piracicaba, Brazil

来源：

JOURNAL OF PLANT PATHOLOGY | 2009年 / 91卷 / 03期

关键词：

Citrus; greening disease; liberibacter; diagnosis; electron microscopy; PCR; GREENING DISEASE; SAO-PAULO; PHYTOPLASMA; SYMPTOMS; BRAZIL; STATE;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Huanlongbing (HLB), the most devastating citrus disease in the world, has been recently seen in Cuba. Characteristic HLB fruit symptoms, blotchy-mottled leaves and yellow shoots were observed on trees of several citrus varieties and species in urban areas of Havana city, and In citrus groves from the central, western and eastern parts of the country The presence of only Ca Liberibacter asiaticus in symptomatic citrus plants and in adult individuals of the Asian citrus psyllid, Diaphorma citri, was determined by polymerase chain reaction (PCR). Transmission electron microscopy showed liberibacter-like structures to be exclusively located in the sieve tubes. DNA fragments amplified from the rplKAJL-rpoBC operon were cloned and sequenced. Sequence analyses revealed 100% identity with the corresponding sequences of ribosomal rpl protein genes from Ca. L. asiaticus.

引用

页码：709 / 712

页数：4

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