Direct ligand screening against membrane proteins on live cells enabled by DNA-programmed affinity labelling

被引：9
|
作者：
Huang, Yiran [1 ,2 ,3 ]
Deng, Yuqing [1 ,2 ,3 ]
Zhang, Jianfu [1 ,2 ,3 ]
Meng, Ling [1 ,2 ,3 ]
Li, Xiaoyu [1 ,2 ,3 ]
机构：
[1] Univ Hong Kong, Dept Chem, Pokfulam Rd, Hong Kong, Peoples R China
[2] Univ Hong Kong, State Key Lab Synthet Chem, Pokfulam Rd, Hong Kong, Peoples R China
[3] Hlth InnoHK, Lab Synthet Chem & Chem Biol, Innovat & Technol Commiss, Hong Kong, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2021年 / 57卷 / 31期
基金：
中国国家自然科学基金;
关键词：
FOLATE RECEPTORS; INHIBITOR; DISCOVERY; CANCER; TECHNOLOGY; CHEMISTRY; ASSAYS;
D O I：
10.1039/d1cc00961c
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Membrane proteins are important drug targets; however, ligand discovery for membrane proteins is highly challenging due to their hydrophobic nature. We show that membrane proteins may be specifically labelled with a DNA tag by DNA-programmed affinity labelling (DPAL), thereby enabling the screening of chemical compounds against membrane proteins directly on live cells.
引用
收藏
页码：3769 / 3772
页数：4
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  • [1] Guiding protein delivery into live cells using DNA-programmed membrane fusion
    Sun, Lele
    Gao, Yanjing
    Wang, Yaoguang
    Wei, Qin
    Shi, Jiye
    Chen, Nan
    Li, Di
    Fan, Chunhai
    [J]. CHEMICAL SCIENCE, 2018, 9 (27) : 5967 - 5975
  • [2] Identification of Histone deacetylase (HDAC)-Associated Proteins with DNA-Programmed Affinity Labeling
    Zhang, Jianfu
    Peng, Jianzhao
    Huang, Yiran
    Meng, Ling
    Li, Qingrong
    Xiong, Feng
    Li, Xiaoyu
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2020, 59 (40) : 17525 - 17532
  • [3] Tuning the Diameter, Stability, and Membrane Affinity of Peptide Pores by DNA-Programmed Self-Assembly
    Fennouri, Aziz
    List, Jonathan
    Ducrey, Julie
    Dupasquier, Jessica
    Sukyte, Viktorija
    Mayer, Simon F.
    Vargas, Reyner D.
    Fernandez, Laura Pascual
    Bertani, Frederick
    Gonzalo, Sandra Rodriguez
    Yang, Jerry
    Mayer, Michael
    [J]. ACS NANO, 2021, 15 (07) : 11263 - 11275
  • [4] Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells
    Huang, Yiran
    Meng, Ling
    Nie, Qigui
    Zhou, Yu
    Chen, Langdong
    Yang, Shilian
    Fung, Yi Man Eva
    Li, Xiaomeng
    Huang, Cen
    Cao, Yan
    Li, Yizhou
    Li, Xiaoyu
    [J]. NATURE CHEMISTRY, 2021, 13 (01) : 77 - +
  • [5] Covalent labelling of fusion proteins in live cells via an engineered receptor-ligand pair
    Krusemark, Casey J.
    Belshaw, Peter J.
    [J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (14) : 2201 - 2204
  • [6] Agonist Discovery for Membrane Proteins on Live Cells by Using DNA-encoded Libraries
    Huang, Yiran
    Hou, Rui
    Lam, Fong Sang
    Jia, Yunxuan
    Zhou, Yu
    He, Xun
    Li, Gang
    Xiong, Feng
    Cao, Yan
    Wang, Dongyao
    Li, Xiaoyu
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2024, 146 (35) : 24638 - 24653
  • [7] Ligand-Directed Acyl Imidazole Chemistry for Labeling of Membrane-Bound Proteins on Live Cells
    Fujishima, Sho-hei
    Yasui, Ryosuke
    Miki, Takayuki
    Ojida, Akio
    Hamachi, Itaru
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (09) : 3961 - 3964
  • [8] Ligand Screening of Membrane Proteins Embedded in Nanodiscs: How to Manage Non-Specific Interactions in Weak Affinity Chromatography?
    Vidal, Francois-Xavier
    Deloche, Adrien
    Zeder-Lutz, Gabrielle
    Hideux, Maria
    Wagner, Renaud
    Dugas, Vincent
    Demesmay, Claire
    [J]. MOLECULES, 2024, 29 (12):
  • [9] Cells and cell lysates: A direct approach for engineering antibodies against membrane proteins using yeast surface display
    Tillotson, Benjamin J.
    Cho, Yong Ku
    Shusta, Eric V.
    [J]. METHODS, 2013, 60 (01) : 27 - 37
  • [10] Going native: Direct high throughput screening of secreted full-length IgG antibodies against cell membrane proteins
    Fang, Yongliang
    Chu, Thach H.
    Ackerman, Margaret E.
    Griswold, Karl E.
    [J]. MABS, 2017, 9 (08) : 1253 - 1261
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