Fyn and CD70 Expression in CD4+T Cells from Patients with Systemic Lupus Erythematosus

被引:9
|
作者
Kozlowska, Anna [1 ]
Hrycaj, Pawel
Lacki, Jan K.
Jagodzinski, Pawel P. [1 ]
机构
[1] Poznan Univ Med Sci, Dept Biochem & Mol Biol, Poznan, Poland
关键词
SYSTEMIC LUPUS ERYTHEMATOSUS; CD70; EXPRESSION; CD4+T CELLS; T-CELLS; SIGNALING PATHWAYS; REVISED CRITERIA; DNA METHYLATION; IL-2; PRODUCTION; KINASE; RECEPTOR; OVEREXPRESSION; CLASSIFICATION; LYMPHOCYTES;
D O I
10.3899/jrheum.090424
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective. CD4+ T cells from patients with systemic lupus erythematosus (SLE) display defective function that contributes to abnormal activation of B cells and autoantibody production. Methods. We compared the transcript and protein levels of Fyn and CD70 in CD4+ T cells from patients with SLE (n = 41) and healthy individuals (n = 34). The CD4+ T cells were isolated by positive biomagnetic separation technique. The quantitative analysis of messenger RNA was performed by reverse transcription and real-time quantitative PCR. The protein contents in the CD4+ T cells were determined by Western blotting analysis. Results. We observed significantly higher levels of Fyn (p = 0.03) and CD70 (p = 0.029) transcripts in SLE CD4+ T cells than in controls. There was a significant increase in CD70 protein levels (p < 0.0001), but not Fyn protein levels (p = 0.081) in CD4+ T cells from patients with SLE compared to healthy individuals. In the group with high disease activity [SLE Disease Activity Index (SLEDAI) >= 9], we observed a significantly higher Fyn protein content than in controls (p = 0.030). There was no correlation between Fyn and CD70 protein levels in SLE CD4+ T cells and disease activity as expressed in the SLEDAI scale. Conclusion. We confirmed previous observations of higher expression of CD70 in CD4+ T cells from patients with SLE. Our findings suggest that increased Fyn protein content in CD4+ T cells can be associated with high SLE disease activity. (First Release Dec 1 2009; J Rheumatol 2010;37:53-9; doi:10.3899/jrheum.090424)
引用
收藏
页码:53 / 59
页数:7
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