Protective Effects of Lactoferrin against SARS-CoV-2 Infection In Vitro

被引：82
|
作者：
Salaris, Claudio [1 ]
Scarpa, Melania [2 ]
Elli, Marina [3 ]
Bertolini, Alice [1 ]
Guglielmetti, Simone [4 ]
Pregliasco, Fabrizio [5 ]
Blandizzi, Corrado [6 ]
Brun, Paola [1 ]
Castagliuolo, Ignazio [1 ]
机构：
[1] Univ Padua, Dept Mol Med, I-35121 Padua, Italy
[2] Veneto Inst Oncol IOV IRCCS, Lab Adv Translat Res, I-35128 Padua, Italy
[3] AAT Adv Analyt Technol Srl, I-29122 Fiorenzuola Darda, Piacenza, Italy
[4] Univ Milan, Dept Food Environm & Nutr Sci DeFENS, I-20122 Milan, Italy
[5] Univ Milan, IRCCS Ist Ortoped Galeazzi, I-20136 Milan, Italy
[6] Univ Pisa, Dept Clin & Expt Med, Unit Pharmacol & Pharmacovigilance, I-56126 Pisa, Italy
来源：
NUTRIENTS | 2021年 / 13卷 / 02期
关键词：
lactoferrin; SARS-CoV-2; antiviral immunity; COVID-19;
D O I：
10.3390/nu13020328
中图分类号：
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号：
100403 ;
摘要：
SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.
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页码：1 / 12
页数：12
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