Effect of oxygen tension on the developmental potential of parthenogenetic oocytes and nuclear-transferred porcine oocytes receiving fetal fibroblast cells

被引:5
|
作者
Kawakami, M [1 ]
Tani, T [1 ]
Yin, XJ [1 ]
Kato, Y [1 ]
Tsunoda, Y [1 ]
机构
[1] Kinki Univ, Coll Agr, Lab Anim Reprod, Nara 6318505, Japan
来源
关键词
porcine; nuclear transfer; oxygen tension; parthenogenones; somatic cells;
D O I
10.1262/jrd.48.409
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The effects of oxygen tension (5% and 20%) during in vitro culture of oocytes and fetal fibroblast cells on the developmental potential of parthenogenetic and nuclear-transferred porcine oocytes were examined. The potential of parthenogenetic oocytes matured and cultured under different oxygen tensions to develop into blastocysts was not significantly different (5 to 16%). The proportions of enucleated oocytes receiving fetal fibroblast cells that developed into blastocysts were significantly lower when somatic cells were cultured under low oxygen tension (2 and 3%) than under high oxygen tension (7%). The effects of oxygen tension during culture of oocytes and somatic cells on the developmental potential of parthenogenetic and nuclear-transferred oocytes is discussed.
引用
收藏
页码:409 / 414
页数:6
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