Rapid determination of cadmium in rice by immunochromatography using anti-(Cd-EDTA) antibody labeled with gold particle

被引：10

作者：

Sasaki, Kazuhiro

Tawarada, Kei

Okuyama, Akira

Kayama, Fujio

Abe, Kaoru

Okuhata, Hiroshi

Maruyama, Yukinao

Arakane, Tamami

Miyasaka, Hitoshi

Fujikawa, Takashi

Ohmura, Naoya

机构：

[1] Cent Res Inst Elect Power Ind, Abiko, Chiba 2701194, Japan

[2] Kansai Elect Power Co Inc, Seika, Kyoto 6190237, Japan

[3] EnBioTec Labs Co Ltd, Tsukuba, Ibaraki 3050062, Japan

[4] Jichi Med Sch, Shimotsuke, Tochigi 3290498, Japan

[5] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan

[6] Gen Environm Technos Co Ltd, Osaka 5760061, Japan

来源：

BUNSEKI KAGAKU | 2007年 / 56卷 / 01期

关键词：

cadmium; immunochromatography; immunoassay; brown rice; Cd-EDTA;

D O I：

10.2116/bunsekikagaku.56.29

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and rapid immunochromatography (IC) system for the detection of Cd in rice has been developed. The produced anti-Cd-EDTA antibody exhibited the equilibrium dissociation constant (K-d) for Cd-EDTA at 1.3 x 10(-8) M, which was almost 100-fold tighter than K-d for the other tested EDTA-metal complex. Cadmium in rice was extracted by HCl, and the extract was introduced into a column to remove co-existing metals (such as zinc, manganese and magnesium) while retaining cadmium. After a pretreatment, IC could detect cadmium in the range from 0.01 to 0.1 mg L-1. Fifty brown rice samples were tested with the column treatment and the IC assay. The estimated cadmium concentrations from the assay were evaluated by a comparison with the results of a nitrate treatment and ICP-AES analysis for the samples. Two measurements were highly correlated, with a correlation coefficient of 0.94.

引用

页码：29 / 36

页数：8