The poor regenerative ability of injured tendon tissues remains a clinical challenge. However, decellularized extracellular matrix (ECM) combined with stem cells shows promise. In contrast to bovine and porcine ECM, marine-derived decellularized ECM has several advantages; it is easily obtained, poses less biological risk, and is not contraindicated on religious grounds. This study successfully fabricated decellularized tilapia fish skin (DTFS) with copious preserved collagen fibers and natural pore structures. The outer layer is smooth and dense, while the inner layer has a soft structure with a rough surface. After crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS), crosslinked DTFS (C-DTFS) showed improved mechanics in dry and wet conditions. In vitro, the leach liquor of crosslinked DTFS showed no cytotoxicity and promoted migration and tenonic differentiation of tendon-derived stem cells (TDSCs). Meanwhile, TDSCs seeded in the inner surface of DTFS maintained viability, differentiated, and exhibited spreading. Furthermore, cell-seeded scaffolds guided the regeneration of tendon tissue in a rat Achilles tendon defect model. Our results suggest that DTFS combined with TDSCs is a novel and promising therapeutic option for tendon tissue engineering.
机构:
Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Ning, Liang-Ju
Zhang, Yi
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Zhang, Yi
Chen, Xiao-He
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Chen, Xiao-He
Luo, Jing-Cong
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Luo, Jing-Cong
Li, Xiu-Qun
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Li, Xiu-Qun
Yang, Zhi-Ming
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Yang, Zhi-Ming
Qin, Ting-Wu
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Sichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
Sichuan Univ, Regenerat Med Res Ctr, W China Hosp, Chengdu 610041, Sichuan, Peoples R ChinaSichuan Univ, Inst Stem Cell & Tissue Engn, State Key Lab Biotherapy, W China Hosp, Chengdu 610041, Sichuan, Peoples R China
机构:
Indian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, IndiaIndian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, India
Ruhela, Aakanksha
Bhatt, Akshay
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Indian Inst Technol Hyderabad, Dept Biomed Engn, Regenerat Med & Stem Cell Lab RMS, Kandi, Telangana, IndiaIndian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, India
Bhatt, Akshay
Rath, Subha Narayan
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Indian Inst Technol Hyderabad, Dept Biomed Engn, Regenerat Med & Stem Cell Lab RMS, Kandi, Telangana, India
Indian Inst Technol Hyderabad, Dept Biomed Engn, Regenerat Med & Stem cell RMS Lab, Kandi 502285, Telangana, IndiaIndian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, India
Rath, Subha Narayan
Sharma, Chandra Shekhar
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Indian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, India
Indian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi 502285, Telangana, IndiaIndian Inst Technol Hyderabad, Dept Chem Engn, Creat & Adv Res Based Nanomat CARBON Lab, Kandi, Telangana, India