Decellularized tilapia fish skin: A novel candidate for tendon tissue engineering

被引:14
|
作者
Liu, Zhe [1 ]
Yu, Ming-Zhao [1 ]
Peng, Hao [1 ]
Liu, Ruo-Tao [1 ]
Lim, Thou [1 ]
Zhang, Chang-Qing [1 ,2 ]
Zhu, Zhen-Zhong [1 ]
Wei, Xiao-Juan [2 ]
机构
[1] Shanghai Jiao Tong Univ, Dept Orthoped Surg, Shanghai Peoples Hosp 6, Sch Med, 600 Yishan Rd, Shanghai 200233, Peoples R China
[2] Shanghai Jiao Tong Univ, Inst Microsurg Extrem, Shanghai Peoples Hosp 6, Sch Med, 600 Yishan Rd, Shanghai 200233, Peoples R China
基金
中国国家自然科学基金;
关键词
Decellularized tilapia fish skin; Tendon tissue engineering; Tendon derived stem cells; Tenonic differentiation; Achilles tendon defect; ACHILLES-TENDON; CROSS-LINKING; COLLAGEN; SCAFFOLDS; INTEGRIN; REPAIR; EXPRESSION; ADHESION; LIGAMENT; MATRIX;
D O I
10.1016/j.mtbio.2022.100488
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The poor regenerative ability of injured tendon tissues remains a clinical challenge. However, decellularized extracellular matrix (ECM) combined with stem cells shows promise. In contrast to bovine and porcine ECM, marine-derived decellularized ECM has several advantages; it is easily obtained, poses less biological risk, and is not contraindicated on religious grounds. This study successfully fabricated decellularized tilapia fish skin (DTFS) with copious preserved collagen fibers and natural pore structures. The outer layer is smooth and dense, while the inner layer has a soft structure with a rough surface. After crosslinking with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS), crosslinked DTFS (C-DTFS) showed improved mechanics in dry and wet conditions. In vitro, the leach liquor of crosslinked DTFS showed no cytotoxicity and promoted migration and tenonic differentiation of tendon-derived stem cells (TDSCs). Meanwhile, TDSCs seeded in the inner surface of DTFS maintained viability, differentiated, and exhibited spreading. Furthermore, cell-seeded scaffolds guided the regeneration of tendon tissue in a rat Achilles tendon defect model. Our results suggest that DTFS combined with TDSCs is a novel and promising therapeutic option for tendon tissue engineering.
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页数:13
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