Labrys soli sp nov., isolated from the rhizosphere of ginseng

In this study, we describe strain DCY64(T) that was isolated from the rhizosphere of three-year-old Korean ginseng root. Cells were Gram-reaction negative, oxidase-and catalase-positive, strictly aerobic, capsulated, non-motile, non-sporulating and spherical to short rod-shaped. Multiplicative budding cells were produced. Vesicles covered the surface of cells. Phylogenetic analysis placed strain DCY64(T) within the genus Labrys with the highest similarity to Labrys monachus VKM B-1479(T) (97.6% 16S rRNA gene sequence similarity), followed by Labrys okinawensis MAFF 210191(T) (97.5 %), Labrys miyagiensis G24103(T) (97.4) and Labrys portucalensis F11(T) (97.0 %). The genomic DNA G+C content was 63 mol%. The presences of summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c), C-19 : 1 cyclo omega 8c and C-16 : 0 as major fatty acids; phosphatidylmonomethylethanolamine, phosphatidylglycerol, phosphatidylcholine and diphosphatidylglycerol as major polar lipids; ubiquinone Q-10 as the predominant quinone and sym-homospermidine as the dominant polyamine were found in strain DCY64(T). These chemotaxonomic results were in accordance with those of members of the genus Labrys. However, the absence of C-16 : 0 2-OH, C-16 : 0 3-OH and C-18 : 1 2-OH from the fatty acids profile and differences in minor polar lipids and phenotypic characteristics distinguished strain DCY64(T) from the closest type strains. The discrimination was also supported by unique enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) fingerprints, as well as DNA-DNA hybridization values (<= 48 %) between strain DCY64(T) and related type strains. Therefore, we propose that strain DCY64(T) represents a novel species of the genus Labrys. The name Labrys soli sp. nov. is proposed, with DCY64(T) (=KCTC 32173(T)=JCM 19895(T)) as the type strain.