Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes

被引：34

作者：

Biderre-Petit, Corinne ^{[1
,2
]}

Dugat-Bony, Eric ^{[3
]}

Mege, Mickael ^{[1
,2
]}

Parisot, Nicolas ^{[1
,2
,4
]}

Adrian, Lorenz ^{[5
]}

Mone, Anne ^{[1
,2
]}

Denonfoux, Jeremie ^{[4
]}

Peyretaillade, Eric ^{[4
]}

Debroas, Didier ^{[1
,2
]}

Boucher, Delphine ^{[4
]}

Peyret, Pierre ^{[4
]}

机构：

[1] Univ Blaise Pascal, Clermont Univ, Lab Microorganismes Genome & Environm, F-63000 Clermont Ferrand, France

[2] CNRS, UMR 6023, Lab Microorganismes Genome & Environm, F-63171 Aubiere, France

[3] Univ Paris Saclay, INRA, AgroParisTech, UMR GMPA, F-78850 Thiverval Grignon, France

[4] Clermont Univ, Univ Auvergne, EA CIDAM 4678, F-63001 Clermont Ferrand, France

[5] UFZ Helmholtz Ctr Environm Res, D-04318 Leipzig, Germany

来源：

PLOS ONE | 2016年 / 11卷 / 01期

关键词：

POLYCHLORINATED-BIPHENYLS; MICROBIAL COMMUNITIES; VERTICAL-DISTRIBUTION; GENOME SEQUENCE; LAC PAVIN; TETRACHLOROETHENE; DIVERSITY; SEDIMENTS; BACTERIA; BIODEGRADATION;

D O I：

10.1371/journal.pone.0145558

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Here we describe the natural occurrence of bacteria of the class Dehalococcoidia (DEH) and their diversity at different depths in anoxic waters of a remote meromictic lake (Lake Pavin) using 16S rRNA gene amplicon sequencing and quantitative PCR. Detected DEH are phylogenetically diverse and the majority of 16S rRNA sequences have less than 91% similarity to previously isolated DEH 16S rRNA sequences. To predict the metabolic potential of detected DEH subgroups and to assess if they encode genes to transform halogenated compounds, we enriched DEH-affiliated genomic DNA by using a specific-gene capture method and probes against DEH-derived 16S rRNA genes, reductive dehalogenase genes and known insertion sequences. Two reductive dehalogenase homologous sequences were identified from DEH-enriched genomic DNA, and marker genes in the direct vicinity confirm that gene fragments were derived from DEH. The low sequence similarity with known reductive dehalogenase genes suggests yet-unknown catabolic potential in the anoxic zone of Lake Pavin.

引用

页数：19