Mediation of protein kinase C zeta in μ-opioid receptor activation for increase of glucose uptake into cultured myoblast C2C12 cells

被引：15
|
作者：
Yang, Ting-Ting [2 ]
Liu, I-Min [3 ,4 ]
Wu, Hung-Tsung [1 ]
Cheng, Juei-Tang [1 ,5 ]
机构：
[1] Natl Cheng Kung Univ, Inst Basic Med Sci, Coll Med, Dept Pharmacol, Tainan 70101, Taiwan
[2] China Med Univ, Coll Med, Grad Inst Basic Med Sci, Taichung 40401, Taiwan
[3] Tajen Univ, Dept Pharm, Yen Pou 90701, Ping Tung Shien, Taiwan
[4] Tajen Univ, Grad Inst Pharmaceut Technol, Yen Pou 90701, Ping Tung Shien, Taiwan
[5] Chi Mei Med Ctr, Dept Med Res, Yung Kang 73101, Tainan Hsien, Taiwan
来源：
NEUROSCIENCE LETTERS | 2009年 / 465卷 / 02期
关键词：
C2C12; cells; Loperamide; mu-Opioid receptors; Protein kinase C-zeta; INDUCED DIABETIC-RATS; HUMAN SKELETAL-MUSCLE; TRANSPORT;
D O I：
10.1016/j.neulet.2009.08.026
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The present Study is designed to investigate the role of atypical protein kinase C(PKC) in the signaling of mu-opioid receptors (MOR) for glucose uptake in myoblast C2C12 cells Loperamide enhanced the uptake of radioactive deoxyglucose into C2C12 Cells in a concentration-dependent manner that was abolished in cells pre-incubated with GF109203X at concentrations sufficient to block PKC Inhibition of the atypical zeta (zeta) isoform of PKC using myristoylated PKC pseudosubstrate resulted in a concentration-dependent decrease of loperamide-stimulated glucose uptake into C2C12 cells In addition, loperamide elicited the phosphorylation of PKC-zeta in C2C12 cells in a concentration-dependent manner that was abolished by pretreatment with naloxonazine at concentrations sufficient to block MOR. These results suggest the mediation of PKC-zeta in MOR signaling for glucose uptake in C2C12 cells. Activation of PKC-zeta by MOR stimulation is highly relevant to the search for therapeutic targets for glucose transport in insulin-sensitive tissues (c) 2009 Elsevier Ireland Ltd. All rights reserved
引用
收藏
页码：177 / 180
页数：4
相关论文
共 50 条
  • [1] Activation of α1A-adrenoceptor by andrographolide to increase glucose uptake in cultured myoblast C2C12 cells
    Hsu, JH
    Liou, SS
    Yu, BC
    Cheng, JT
    Wu, YC
    PLANTA MEDICA, 2004, 70 (12) : 1230 - 1233
  • [2] Signals in the activation of opioid μ-receptors by loperamide to enhance glucose uptake into cultured C2C12 cells
    Liou, SS
    Chen, WC
    Chen, PF
    Cheng, JT
    HORMONE AND METABOLIC RESEARCH, 2004, 36 (04) : 210 - 214
  • [3] Activation of imidazoline I2B receptors is linked with AMP kinase pathway to increase glucose uptake in cultured C2C12 cells
    Lui, Tai-Ngar
    Tsao, Chiung-Wen
    Huang, Shih-Yun
    Chang, Chin-Hong
    Cheng, Juei-Tang
    NEUROSCIENCE LETTERS, 2010, 474 (03) : 144 - 147
  • [4] Characterization of adenosine A1 receptor in cultured myoblast C2C12 cells of mice
    Liu, IM
    Lai, TY
    Tsai, CC
    Cheng, JT
    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, 2001, 87 (01): : 59 - 64
  • [5] Stimulatory effect of isoferulic acid on α1A-adrenoceptor to increase glucose uptake into cultured myoblast C2C12 cell of mice
    Liu, IM
    Tsai, CC
    Lai, TY
    Cheng, JT
    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, 2001, 88 (03): : 175 - 180
  • [6] Activation of Imidazoline I-2B Receptors by Allantoin to Increase Glucose Uptake into C2C12 Cells
    Chen, M. F.
    Yang, T. T.
    Yeh, L. R.
    Chung, H. H.
    Wen, Y. J.
    Lee, W. J.
    Cheng, J. T.
    HORMONE AND METABOLIC RESEARCH, 2012, 44 (04) : 268 - 272
  • [7] Stimulatory effect of caffeic acid on α1A-adrenoceptors to increase glucose uptake into cultured C2C12 cells
    Cheng, JT
    Liu, IM
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2000, 362 (02) : 122 - 127
  • [8] Stimulatory effect of caffeic acid on α1A-adrenoceptors to increase glucose uptake into cultured C2C12 cells
    Juei-Tang Cheng
    I-Min Liu
    Naunyn-Schmiedeberg's Archives of Pharmacology, 2000, 362 : 122 - 127
  • [9] Stimulatory effect of puerarin on α1A-adrenoceptor to increase glucose uptake into cultured C2C12 cells of mice
    Hsu, HH
    Chang, CK
    Su, HC
    Liu, IM
    Cheng, JT
    PLANTA MEDICA, 2002, 68 (11) : 999 - 1003
  • [10] Leptin stimulates glucose uptake in C2C12 muscle cells by activation of ERK2
    Berti, L
    Gammeltoft, S
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1999, 157 (1-2) : 121 - 130
12345