Effects of Gold Nanoparticles with Different Surface Charges on Cellular Internalization and Cytokine Responses in Monocytes

被引:10
|
作者
Srijampa, Sukanya [1 ]
Buddhisa, Surachat [2 ]
Ngernpimai, Sawinee [3 ]
Sangiamdee, Duangkamon [4 ]
Chompoosor, Apiwat [4 ]
Tippayawat, Patcharaporn [1 ,5 ]
机构
[1] Khon Kaen Univ, Fac Associated Med Sci, Ctr Res & Dev Med Diagnost Labs, Biosensor Res Grp Noncommunicable Dis & Infect Di, Khon Kaen 40002, Thailand
[2] Univ Phayao, Fac Allied Hlth Sci, Dept Med Technol, Phayao 56000, Thailand
[3] Khon Kaen Univ, Mat Sci & Nanotechnol Program, Fac Sci, Khon Kaen 40002, Thailand
[4] Ramkhamhang Univ, Fac Sci, Dept Chem, Ramkhamhaeng Rd, Bangkok 10240, Thailand
[5] Khon Kaen Univ, Fac Associated Med Sci, Dept Med Technol, Khon Kaen 40002, Thailand
关键词
Surface charge; Gold nanoparticles; Cell uptake pathways; Immunity; SIZE; PATHWAY; CYTOTOXICITY; PARTICLES;
D O I
10.1007/s12668-019-00638-8
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Surface modification of gold nanoparticles (AuNPs) affects the interactions between the AuNPs and biological systems. To use AuNPs in biomedical applications, an investigation of how different surface charges of AuNPs affect the internalization mechanisms and functions of monocytes is needed. In this study, U937 cells were exposed to AuNPs with positive (AuNPs+ve) and negative (AuNPs-ve) surface charges. Cellular responses to the AuNPs, including cell uptake pathways, cell activation and cytokine production, were analysed. The results indicated different responses to AuNPs-ve and AuNPs+ve in outcomes including internalization pathways, cell activation and cytokine production levels. Interestingly, the results suggested that AuNPs+ve tended to enter the cells via macropinocytosis and strongly elicited a cellular response of IL-6 production via NF-kB activation, whereas the AuNPs-ve suppressed IL-6 production. Therefore, these results provide essential information for designing nanoparticles and may contribute to possible future nanomedicine applications, such as drug delivery and vaccination.
引用
收藏
页码:580 / 586
页数:7
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