Comparison of various extraction techniques for isolation and determination of isoflavonoids in plants

被引:37
|
作者
Bajer, Tomas [1 ]
Adam, Martin [1 ]
Galla, Lubomir [1 ]
Ventura, Karel [1 ]
机构
[1] Univ Pardubice, Dept Analyt Chem, Fac Chem Technol, Pardubice 53210, Czech Republic
关键词
extraction; HPLC; isoflavonoids;
D O I
10.1002/jssc.200600306
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In the present paper, the following extraction techniques have been used for extracting isoflavonoids from the species Matricaria recutita, Rosmarinus officinalis, Foeniculum vulgare, and Agrimonia eupatoria L.: supercritical fluid extraction (SFE), pressurized fluid extraction, matrix solid phase dispersion, ultrasonic extraction in an ultrasonic bath (USE) and by means of an ultrasonic homogeniser (HOM), extraction by means of Soxhlet apparatus (SOX), and solid phase extraction. Experimental optimization of all techniques has been carried out using a soybean flour. Subsequent analyses of the extracts were carried out by liquid chromatography with UV detection. The maximum yields of daidzein and genistein were obtained by extraction with the SOX, USE, and HOM techniques. The maximum yields of apigenin and biochanin A from herb samples were obtained by SFE.
引用
收藏
页码:122 / 127
页数:6
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