Discriminatory power of rbcL barcode locus for authentication of some of United Arab Emirates (UAE) native plants

被引:38
|
作者
Maloukh, Lina [1 ]
Kumarappan, Alagappan [1 ]
Jarrar, Mohammad [1 ]
Salehi, Jawad [1 ]
El-Wakil, Houssam [1 ]
Lakshmi, T. V. Rajya [1 ]
机构
[1] Univ Modern Sci, Coll Biotechnol, Al Twar 3,POB 231931, Dubai, U Arab Emirates
基金
新加坡国家研究基金会;
关键词
United Arab Emirates; Monocots; Eudicots; Native plants; rbcL; matK; Barcode; DNA BARCODES; MATK; IDENTIFICATION; TAXONOMY; REGIONS; GENE;
D O I
10.1007/s13205-017-0746-1
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
DNA barcoding of United Arab Emirates (UAE) native plants is of high practical and scientific value as the plants adapt to very harsh environmental conditions that challenge their identification. Fifty-one plant species belonged to 22 families, 2 monocots, and 20 eudicots; a maximum number of species being legumes and grasses were collected. To authenticate the morphological identification of the wild plant taxa, rbcL and matK regions were used in the study. The primer universality and discriminatory power of rbcL is 100%, while it is 35% for matK locus for these plant species. The sequences were submitted to GenBank; accession numbers were obtained for all the rbcL sequences and for 6 of matK sequences. We suggest rbcL as a promising barcode locus for the tested group of 51 plants. In the present study, an inexpensive, simple method of identification of rare desert plant taxa through rbcL barcode is being reported.
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页数:7
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