Imaging growth dynamics of tumour spheroids using optical coherence tomography

被引：26

作者：

Sharma, M. ^{[1
]}

Verma, Y. ^{[1
]}

Rao, K. D. ^{[1
]}

Nair, R. ^{[1
]}

Gupta, P. K. ^{[1
]}

机构：

[1] Raja Ramanna Ctr Adv Technol, Biomed Applicat Sect, Indore 452013, India

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 02期

关键词：

growth dynamics; optical coherence tomography; tumour spheroids;

D O I：

10.1007/s10529-006-9232-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Optical coherence tomography (OCT) was used to monitor the dynamics of tumour spheroid formation by the hanging drop method. In contrast to microscopy, the estimates obtained using OCT for the volume of the spheroid, were consistent with the measured changes in cell number as a function of time. The OCT images also revealed heterogeneous structures in the spheroids of similar to 200 mu m diameter. These corresponded to the necrotic regions identified by fluorescence of propidium iodide stained cells.

引用

页码：273 / 278

页数：6

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