Interaction of ectoine and hydroxyectoine with protein: fluorescence study

被引：9
|
作者：
Rasteniene, Auguste [1 ]
Gruskiene, Ruta [1 ]
Sereikaite, Jolanta [1 ]
机构：
[1] Vilnius Gediminas Tech Univ, Fac Fundamental Sci, Dept Chem & Bioengn, Sauletekio Al 11, LT-10223 Vilnius 40, Lithuania
来源：
CHEMICAL PAPERS | 2021年 / 75卷 / 06期
关键词：
Ectoine; Hydroxyectoine; Mink growth hormone; Acrylamide; Fluorescence quenching; Sphere action static mechanism;
D O I：
10.1007/s11696-021-01527-9
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Ectoine and hydroxyectoine are well known extremolytes that accumulate in halophilic and halotolerant microorganisms. They are investigated as protein stabilizers and as excipients in protein formulations and biopharmaceuticals. However, their effect is quite controversial. In this work, the influence of both extremolytes on mink growth hormone as a model protein was studied by fluorescence spectroscopy. The study revealed that the fluorescence quenching of protein tryptophan by ectoine and hydroxyectoine differs and is dependent on the pH value of solution. Moreover, the quenching mode by ectoine and hydroxyectoine was compared with a well-known quencher acrylamide. The differences in the extremolytes interaction with protein revealed by fluorescence spectroscopy could be related to their different effect as protein stabilizers under various conditions.
引用
收藏
页码：2703 / 2712
页数：10
相关论文
共 50 条
  • [1] Interaction of ectoine and hydroxyectoine with protein: fluorescence study
    Auguste Rasteniene
    Ruta Gruskiene
    Jolanta Sereikaite
    Chemical Papers, 2021, 75 : 2703 - 2712
  • [2] Hydration in aqueous solutions of ectoine and hydroxyectoine
    Sahle, Christoph J.
    Schroer, Martin A.
    Jeffries, Cy M.
    Niskanen, Johannes
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2018, 20 (44) : 27917 - 27923
  • [3] BIOSYNTHESIS AND THE POSSIBILITY OF USING ECTOINE AND HYDROXYECTOINE IN HEALTH CARE
    Goraj, Weronika
    Stepniewska, Zofia
    Szafranek-Nakonieczna, Anna
    ADVANCEMENTS OF MICROBIOLOGY, 2019, 58 (03) : 339 - 350
  • [4] Metabolic profiling of ectoine and hydroxyectoine production by Chromohalobacter salexigens
    Canovas, M.
    Pastor, J. M.
    Argandona, M.
    Vergas, C.
    Nieto, J. J.
    Iborra, J. L.
    NEW BIOTECHNOLOGY, 2009, 25 : S341 - S341
  • [5] The effect of extremolytes ectoine and hydroxyectoine on the heat-induced protein aggregation: The case of growth hormone
    Gruskiene, Ruta
    Sereikaite, Jolanta
    BIOCHIMIE, 2025, 229 : 42 - 48
  • [6] Microbial production of ectoine and hydroxyectoine as high-value chemicals
    Liu, Mengshuang
    Liu, Hui
    Shi, Meng
    Jiang, Mingyue
    Li, Lingling
    Zheng, Yanning
    MICROBIAL CELL FACTORIES, 2021, 20 (01)
  • [7] Microbial production of ectoine and hydroxyectoine as high-value chemicals
    Mengshuang Liu
    Hui Liu
    Meng Shi
    Mingyue Jiang
    Lingling Li
    Yanning Zheng
    Microbial Cell Factories, 20
  • [8] Ectoine and hydroxyectoine inhibit aggregation and neurotoxicity of Alzheimer's β-amyloid
    Kanapathipillai, M
    Lentzen, G
    Sierks, M
    Park, CB
    FEBS LETTERS, 2005, 579 (21) : 4775 - 4780
  • [9] Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture
    Tsai, Teresa
    Mueller-Buehl, Ana M.
    Satgunarajah, Yathavan
    Kuehn, Sandra
    Dick, H. Burkhard
    Joachim, Stephanie C.
    GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2020, 258 (10) : 2185 - 2203
  • [10] Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture
    Teresa Tsai
    Ana M. Mueller-Buehl
    Yathavan Satgunarajah
    Sandra Kuehn
    H. Burkhard Dick
    Stephanie C. Joachim
    Graefe's Archive for Clinical and Experimental Ophthalmology, 2020, 258 : 2185 - 2203
12345