Ecophysiology of Fusarium temperatum isolated from maize in Argentina

The effect of water activity (a(w) = 0.95, 0.98 and 0.995), temperature (15, 25 and 30 degrees C), incubation time (7, 14, 21 and 28days), and their interactions on growth and moniliformin (MON), beauvericin (BEA), fusaproliferin (FUS) and fumonisin B-1 (FB1) production by two strains of Fusarium temperatum isolated from Argentinean maize were determined in vitro on sterile layers of maize grains. The results showed that there was a wide range of conditions for growth and mycotoxins production by F. temperatum. Both strains were found to grow faster with increasing a(w) and at 30 degrees C. In relation to mycotoxin production, the two strains produced more FUS than the other mycotoxins regardless of a(w) or temperature evaluated (maximum = 50000gg(-1)). For FUS, MON and BEA, the maximum levels were observed at 0.98 a(w) and 30 degrees C (50000, 5000 and 2000gg(-1) respectively). The lowest levels for these three mycotoxins were detected at 15 degrees C and 0.95 a(w) (1700 and 100gg(-1) for FUS and MON respectively), and at 0.98 a(w) (400gg(-1) for BEA). The maximum levels of FB1 were produced at 15 degrees C and 0.98 a(w) (1000 gg(-1)). At all a(w) and temperatures combinations evaluated there was an increase in toxin concentrations with time incubation. The maximum levels were detected at 21days. Statistical analyses of a(w), temperature, incubation time, and the two- and three-way interactions between them showed significant effects on mycotoxins production by F. temperatum. For its versatility on growth and mycotoxin production, F. temperatum represents a toxicological risk for maize in the field and also during grain storage.