Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue

被引：14
|
作者：
Romanov, Yu. A. [1 ,3 ]
Balashova, E. E. [1 ,2 ]
Volgina, N. E. [2 ]
Kabaeva, N. V. [1 ]
Dugina, T. N. [2 ]
Sukhikh, G. T. [2 ]
机构：
[1] Minist Hlth Russian Federat, Russian Cardiol Res & Prod Complex, Moscow, Russia
[2] Minist Hlth Russian Federat, VI Kulakov Res Ctr Obstet Gynecol & Perinatol, Moscow, Russia
[3] CryoCtr Umbil Cord Blood Bank, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2016年 / 160卷 / 04期
基金：
俄罗斯科学基金会;
关键词：
multipotent mesenchymal stromal cells; umbilical cord; cryopreservation; cell culture; STEM-CELLS; CAPACITY;
D O I：
10.1007/s10517-016-3213-9
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies.
引用
收藏
页码：530 / 534
页数：5
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