Synthesis of Threefold Glycosylated Proteins using Click Chemistry and Genetically Encoded Unnatural Amino Acids

被引：49

作者：

Kaya, Emine ^{[1
]}

Gutsmiedl, Katrin ^{[1
]}

Vrabel, Milan ^{[1
]}

Mueller, Markus ^{[1
]}

Thumbs, Peter ^{[1
]}

Carell, Thomas ^{[1
]}

机构：

[1] Ludwig Maximilians Univ Munchen, Dept Chem & Biochem, Ctr Integrated Prot Sci CiPSM, D-81377 Munich, Germany

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 18期

关键词：

amber suppression; amino acids; click chemistry; protein glycosylation; pyrrolysine; TRANSFER-RNA SYNTHETASE; SITE-SPECIFIC INCORPORATION; NATIVE CHEMICAL LIGATION; GLYCOPROTEIN-SYNTHESIS; RECOMBINANT PROTEINS; TERMINAL ALKYNES; SOLID-PHASE; PYRROLYSINE; CODE; AZIDES;

D O I：

10.1002/cbic.200900625

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Click to convert. Three encoded alkyne and alkene amino acids were incorporated into yellow fluorescent protein (YFP) and subsequently glycosylated by using the CuI catalyzed click reaction, as illustrated in the scheme. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2858 / 2861

页数：4

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