Transfer RNA-derived fragment tRF-28-QSZ34KRQ590K in plasma exosomes may be a potential biomarker for atopic dermatitis in pediatric patients

被引：10

作者：

Meng, Li ^{[1
]}

Jiang, Long ^{[2
]}

Chen, Jian ^{[1
]}

Ren, Hongjin ^{[1
]}

Gao, Zhiqin ^{[1
]}

Wu, Fei ^{[3
]}

Wen, Yiyang ^{[1
]}

Yang, Lianjuan ^{[1
]}

机构：

[1] Tongji Univ, Sch Med, Shanghai Skin Dis Hosp, Dept Dermatol Mycol, 1278 Baode Rd, Shanghai 200443, Peoples R China

[2] Tongji Univ, Sch Med, Shanghai Skin Dis Hosp, Dept Dermatol Surg, Shanghai 200443, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai Skin Dis Hosp, Dept Dermatol Pathol, Shanghai 200443, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2021年 / 21卷 / 05期

基金：

中国国家自然科学基金;

关键词：

atopic dermatitis; biomarker; plasma; exosome; sequence; transfer RNA-derived fragments;

D O I：

10.3892/etm.2021.9920

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Atopic dermatitis (AD) is a common chronic relapsing inflammatory disease. There is substantial evidence suggesting that noncoding RNAs have indispensable roles in the pathogenesis of AD. Exosomal transfer RNA-derived fragments (tRFs) have been identified as potential biomarkers for various disorders. However, the role of tRFs in AD has remained to be elucidated, which was thus the aim of the present study. Plasma samples from 23 pediatric patients with AD and 23 healthy controls were collected. Exosomes were successfully isolated from plasma according to the manufacturer's protocol. Small RNA sequencing was performed in 3 patients with AD and 3 controls, and 135 significantly differentially expressed plasma exosomal tRFs were identified, including 36 upregulated and 99 downregulated tRFs. Using the miRanda and RNAhybrid databases, 58,227 target genes of these 135 differentially expressed tRFs were predicted. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses suggested that these target genes of tRFs are involved in multiple functions and pathways associated with AD. Downregulation of tRF-28-QSZ34KRQ590K and tRF-33-Q99P9P9NH57SD3 were validated in 20 patients with AD and 20 controls by reverse transcription-quantitative PCR and tRF-28-QSZ34KRQ590K exhibited significance in the receiver operating characteristic curve analysis. The present study was the first to provide a tRF profile in AD and implied that plasma exosomal tRF-28-QSZ34KRQ590K may be a potential biomarker for pediatric patients with AD. The present study enhanced the understanding of the pathogenesis of AD and provided a novel marker for the diagnosis and targeted treatment of AD.

引用

页数：7