The use of novel organic gels and hydrogels in protein crystallization

被引：16
|
作者：
Pietras, Zbigniew [1 ]
Lin, Hong-Ting [1 ]
Surade, Sachin [1 ]
Luisi, Ben [1 ]
Slattery, Orla [2 ]
Pos, Klaas M. [2 ]
Moreno, Abel [1 ,3 ]
机构：
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England
[2] Goethe Univ Frankfurt, Inst Biochem, D-60438 Frankfurt, Germany
[3] Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
来源：
JOURNAL OF APPLIED CRYSTALLOGRAPHY | 2010年 / 43卷
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
X-RAY-DIFFRACTION; AGAROSE-GEL; MACROMOLECULAR CRYSTALLIZATION; CRYSTAL-GROWTH; MICROGRAVITY; ACRB; MICROSCOPY; MEDIA;
D O I：
10.1107/S0021889809051917
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The use of an organic solvent-based gel prepared from polyethylene oxide and a polyvinyl alcohol hydrogel for protein crystallization was investigated. The preparation, properties and application of the gels for protein crystallization are described, and the advantages and limitations of the approach are discussed. The gels are compared with agar, which is a popular aqueous gel used for protein crystallization. The growth behaviour and diffraction quality of crystals prepared in these gel media were evaluated for two model soluble proteins, thaumatin and lysozyme, and for two bacterial membrane proteins, TolC and AcrB.
引用
收藏
页码：58 / 63
页数：6
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