HPTLC Densitometric Quantification of Glycyrrhizin, Glycyrrhetinic Acid, Apigenin, Kaempferol and Quercetin from Glycyrrhiza glabra

Glycyrrhiza glabra Linn., commonly known as liquorice, is a reputed drug of Ayurveda. In the present work we developed and validated densitometric methods for quantification of glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin using HPTLC. The developed methods were found to be precise and accurate. The amount of glycyrrhizin, glycyrrhetinic acid, and quercetin was found to be 1.070, 0.84 and 0.271% w/w, respectively. Apigenin and kaempferol were quantified in free (0.007 and 0.033% w/w) as well as bound (0.021 and 0.074% w/w) forms. This is the first report of simultaneous quantification of glycyrrhetinic acid and apigenin as well as kaempferol and quercetin from G. glabra. Furthermore, no TLC densitometric methods have been reported for the quantification of apigenin, kaempferol and quercetin from G. glabra.