High density cell culture of Panax notoginseng for production of ginseng saponin and polysaccharide in an airlift bioreactor

被引：24
|
作者：
Han, J [1 ]
Zhong, JJ [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 22期
基金：
中国国家自然科学基金;
关键词：
airlift bioreactor; ginseng saponin and polysaccharide; high density cell culture; Panax notoginseng; plant cell culture;
D O I：
10.1023/A:1020960410885
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
High cell density of Panax notoginseng in a 17 l airlift bioreactor was achieved in batch cultivation using a modified MS medium. The dry cell weight, ginseng saponin and polysaccharide reached 24, 1.7 and 2.8 g l(-1), respectively, after 15 d. A strategy of sucrose feeding based on changes in the specific O-2 uptake rate was applied to the cell cultures, which increased these respective yields to 30, 2.3 and 3.2 g l(-1).
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页码：1927 / 1930
页数：4
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