Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

被引：36
|
作者：
Jin Ming-hui [1 ,2 ]
Tao Jia-hui [1 ]
Li Q [1 ]
Cheng Ying [1 ]
Sun Xiao-xu [2 ]
Wu Kong-ming [2 ]
Xiao Yu-tao [1 ]
机构：
[1] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen 518120, Peoples R China
[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
来源：
JOURNAL OF INTEGRATIVE AGRICULTURE | 2021年 / 20卷 / 03期
关键词：
Spodoptera frugiperda; ABCC2; CRISPR/Cas9; Bt receptor; Cry1F; HELICOVERPA-ARMIGERA; CRISPR/CAS9; MUTATION; RECEPTOR; CADHERIN; COTTON; MAIZE;
D O I：
10.1016/S2095-3119(19)62772-3
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
ATP-binding cassette transporter C2 (ABCC2) is known to be a receptor for Bacillus thuringiensis (Bt) toxins in several lepidopteran insects. Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1F toxin from Bt in Spodoptera frugiperda. Here we generated a SfABCC2 knockout strain of S. frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1F. Results from bioassays showed that the SfABCC2 knockout S. frugiperda strain displayed 118-fold resistance to Cry1F compared with the parental DH19 strain, but no resistance to Vip3A toxin from Bt. These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1F.
引用
下载
收藏
页码：815 / 820
页数：6
相关论文
共 50 条
  • [1] Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda
    JIN Ming-hui
    TAO Jia-hui
    LI Qi
    CHENG Ying
    SUN Xiao-xu
    WU Kong-ming
    XIAO Yu-tao
    Journal of Integrative Agriculture, 2021, 20 (03) : 815 - 820
  • [2] Individual transmembrane domains of SfABCC2 from Spodoptera frugiperda do not serve as functional Cry1F receptors
    Zhong, Jianfeng
    Dos Santos, Rafael Ferreira
    Abdelgaffar, Heba
    de Bortoli, Caroline Placidi
    Raza, Ahmad
    Jurat-Fuentes, Juan Luis
    PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 2024, 199
  • [3] Geographical and Temporal Variability in Susceptibility to Cry1F Toxin From Bacillus thuringiensis in Spodoptera frugiperda (Lepidoptera: Noctuidae) Populations in Brazil
    Farias, Juliano R.
    Horikoshi, Renato J.
    Santos, Antonio C.
    Omoto, Celso
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2014, 107 (06) : 2182 - 2189
  • [4] Susceptibility of Spodoptera frugiperda (Lepidoptera: Noctuidae) field populations to the Cry1F Bacillus thuringiensis insecticidal protein
    Da Silva, Karen F.
    Spencer, Terence A.
    Crespo, Andre L. B.
    Siegfried, Blair D.
    FLORIDA ENTOMOLOGIST, 2016, 99 (04) : 629 - 633
  • [5] Fitness costs of Cry1F resistance in fall armyworm, Spodoptera frugiperda
    Velez, A. M.
    Spencer, T. A.
    Alves, A. P.
    Crespo, A. L. B.
    Siegfried, B. D.
    JOURNAL OF APPLIED ENTOMOLOGY, 2014, 138 (05) : 315 - 325
  • [6] Frequency of Cry1F resistance alleles in Spodoptera frugiperda (Lepidoptera: Noctuidae) in Brazil
    Farias, Juliano R.
    Andow, David A.
    Horikoshi, Renato J.
    Bernardi, Daniel
    Ribeiro, Rebeca da S.
    do Nascimento, Antonio R. B.
    dos Santos, Antonio C.
    Omoto, Celso
    PEST MANAGEMENT SCIENCE, 2016, 72 (12) : 2295 - 2302
  • [7] Molecular characterization of Spodoptera frugiperda Bacillus thuringiensis Cry1Ca toxin interaction
    Rodriguez-Cabrera, Lianet
    Trujillo-Bacallao, Damian
    Borras-Hidalgo, Orlando
    Wright, Denis J.
    Ayra-Pardo, Camilo
    TOXICON, 2008, 51 (04) : 681 - 692
  • [8] SfABCC2 transporter extracellular loops 2 and 4 are responsible for the Cry1Fa insecticidal specificity against Spodoptera frugiperda
    Liu, Yuanyuan
    Jin, Minghui
    Wang, Ling
    Wang, Haixia
    Xia, Zhichao
    Yang, Yongbo
    Bravo, Alejandra
    Soberon, Mario
    Xiao, Yutao
    Liu, Kaiyu
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2021, 135
  • [9] Cry1F Resistance in Fall Armyworm Spodoptera frugiperda: Single Gene versus Pyramided Bt Maize
    Huang, Fangneng
    Qureshi, Jawwad A.
    Meagher, Robert L., Jr.
    Reisig, Dominic D.
    Head, Graham P.
    Andow, David A.
    Ni, Xinzi
    Kerns, David
    Buntin, G. David
    Niu, Ying
    Yang, Fei
    Dangal, Vikash
    PLOS ONE, 2014, 9 (11):
  • [10] Baseline susceptibility of tobacco budworm (Lepidoptera: Noctuidae) to Cry1F toxin from Bacillus thuringiensis
    Blanco, Carlos A.
    Storer, Nicholas P.
    Abel, Craig A.
    Jackson, Ryan
    Leonard, Rogers
    Lopez, Juan D., Jr.
    Payne, Gregory
    Siegfried, Blair D.
    Spencer, Terence
    Teran-Vargas, Antonio P.
    JOURNAL OF ECONOMIC ENTOMOLOGY, 2008, 101 (01) : 168 - 173
12345