Thermokinetics of activation of metal ions to amylase catalyzed reaction

Power - time curves of amylase catalyzed reaction with or without activation were obtained with a 2277 thermal activity monitor (Sweden). The data of K-m and nu(max) were calculated for enzyme catalyzed reactions without activation. Data of apparent K-m and nu(max) were calculated for activation (Ca2+, Mg2+) using thermokinetics theory and reduced extent method. From the relationship between K-m(1) and concentration of metal ions (c), the effects of the activation were gained for metal ions of amylase catalyzed reaction.