Results of VNTR analysis of locus (5'-CAAA-3')n of Yersinia pestis strains from active natural area of plague in Siberia

被引：0
|
作者：
Balakhonov, S. V. [1 ]
Shestopalov, M. Yu. [1 ]
Romanova, I. F. [1 ]
机构：
[1] Antiplague Res Inst Siberia & Far E, Irkutsk, Russia
来源：
MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY | 2009年 / 24卷 / 03期
关键词：
VNTR-analysis; plaque Yersinia pestis; natural foci; TRIPLET REPEAT;
D O I：
10.3103/S0891416809030045
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA samples of 73 strains of Y. pestis ssp. altaica and 65 strains of Y. pestis ssp. pestis isolated in the Gorno-Altaisk and Tuva natural plague areas, respectively, were investigated by the method of VNTR analysis of a (5'-CAAA-3')n locus. It was demonstrated that strains from the Gorno-Altaisk population of plague microbes possessed genomic polymorphism on the mentioned marker locus, which is reflected in the variability of the size of the amplicon of 250-275 bp, which corresponds to places where the strains were isolated in separate epizootic loci. Conversely, Y. pestis ssp. pestis strains from the Tuva population were conserved by the 5'-CAAA-3' locus (the amplicon size was 261 bp). During the random sequencing of amplicons, the exact sizes and number of 5'-CAAA-3' tetranucleotide repeats were determined.
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页码：128 / 132
页数：5
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