Low cryoprotectant concentration rapid vitrification of mouse oocytes and embryos

被引:7
|
作者
Liu, Jie [1 ,2 ,4 ]
Lee, Gloria Y. [1 ,2 ,4 ]
Biggers, John D. [1 ,2 ,4 ]
Toth, Thomas L. [2 ,3 ,5 ,6 ]
Toner, Mehmet [1 ,2 ,4 ]
机构
[1] Massachusetts Gen Hosp, Dept Surg, Boston, MA 02114 USA
[2] Harvard Med Sch, Boston, MA 02114 USA
[3] Massachusetts Gen Hosp, Dept Obstet & Gynecol, Boston, MA 02114 USA
[4] Shriners Hosp Children, Boston, MA 02114 USA
[5] Boston IVF, Boston, MA 02109 USA
[6] Beth Israel Deaconess Med Ctr, Dept Obstet & Gynecol, Boston, MA 02215 USA
基金
美国国家卫生研究院;
关键词
Vitrification; Low cryoprotectant concentration; Single medium; Mouse; Oocytes; Embryos;
D O I
10.1016/j.cryobiol.2020.10.016
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Vitrification of mammalian oocytes and embryos is typically a two-step procedure involving two solutions of increasing concentrations of cryoprotectants. In the present study, we report a simple vitrification protocol that uses low cryoprotectant concentration and a single medium (LCSM). This medium, along with the traditional high concentration two media (HCTM) protocol, was used to vitrify mouse oocytes, zygotes, and blastocysts using silica capillary, cryotop, cryolock, and 0.25 ml straws. Survival rates, two-cell rates, and blastocyst formation rates were compared for oocytes and zygotes vitrified using both protocols. Results show that the LCSM protocol was as good as or better than the traditional HCTM protocol for vitrifying mouse MII oocytes and zygotes using silica capillary, cryotop, and cryolock. On the other hand, for blastocysts, only silica capillary using LCSM had comparable results with the traditional HCTM protocol while cryolock and cryotop had significantly lower percentages of re-expanded and hatched blastocysts. Collapsing blastocysts prior to vitrification or longer duration for better cryoprotectant distribution in multicellular embryos may improve the outcome. In conclusion, the LCSM protocol, with one medium of much lower cryoprotectant concentrations and shorter equilibration time, reduces exposure to cryoprotectant toxicity while improves efficiency, consistency and reliability for mammalian oocyte and embryo preservation.
引用
收藏
页码:233 / 238
页数:6
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