Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces

被引:168
|
作者
Verdenelli, Maria Cristina [1 ,2 ]
Ghelfi, Francesca [1 ]
Silvi, Stefania [1 ]
Orpianesi, Carla [1 ]
Cecchini, Cinzia [2 ]
Cresci, Alberto [1 ,2 ]
机构
[1] Univ Camerino, Dipartimento Sci Morfol & Biochim Comparate, I-62032 Camerino, Italy
[2] Synbiotec SRL, I-62032 Camerino, Italy
关键词
Lactobacillus rhamnosus; Lactobacillus paracasei; Probiotics; Adhesion; Probiotic combination; Human study; LACTIC-ACID BACTERIA; PLASMID DNA; IN-VITRO; STRAINS; ADHESION; IDENTIFICATION; CELLS; COLONIZATION; POPULATIONS; MICROFLORA;
D O I
10.1007/s00394-009-0021-2
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The possibility of using microbes to maintain health, and to prevent or treat disease is a topic as old as microbiology. The research of novel probiotic strains is important in order to satisfy the increasing request of the market and to obtain functional products in which the probiotic cultures are more active and with better probiotic characteristics than those already present on the market. In this study, the probiotic potential of Lactobacillus strains isolated from Italian elderly human faeces was investigated. The Lactobacillus strains were identified and examined for resistance to gastric acidity and bile toxicity, adhesion to HT-29 cells, antimicrobial activities, antibiotic susceptibility and plasmid profile. Survival of the strains through human intestine was examined in a 3 months human feeding trial. Two strains, Lactobacillus rhamnosus IMC 501 and Lactobacillus paracasei IMC 502, tolerated well low pH and bile acids. In antimicrobial activity assays, both strains showed inhibitory properties towards selected potential harmful microorganisms, particularly against Candida albicans. The two selected strains expressed high in vitro adherence to HT-29 cells increasing this characteristic when they are used in combination and they were resistant to vamcomycin, colistin sulphate, gentamicin, oxolinic acid and kanamycin. Moreover, the two strains could be recovered from stools of volunteers after the feeding trials. Lactobacillus rhamnosus IMC 501 and L. paracasei IMC 502 present favourable strain-specific properties for their utilisation as probiotics in functional foods and the high adhesion ability of the L. rhamnosus IMC 501 and L. paracasei IMC 502 used in combination, confirmed by both in vitro and in vivo study, indicate that the two bacterial strains could be used as health-promoting bacteria.
引用
收藏
页码:355 / 363
页数:9
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