Insect intestinal mucins and serine proteases associated with the peritrophic matrix from feeding, starved and moulting Mamestra configurata larvae

被引：35

作者：

Toprak, U. ^{[1
,2
,3
]}

Baldwin, D. ^{[1
]}

Erlandson, M. ^{[1
,2
]}

Gillott, C. ^{[2
]}

Hegedus, D. D. ^{[1
,4
]}

机构：

[1] Agr & Agri Food Canada, Mol Genet Sect, Saskatoon, SK S7N 0X2, Canada

[2] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 0W0, Canada

[3] Ankara Univ, Coll Agr, Dept Plant Protect, TR-06100 Ankara, Turkey

[4] Univ Saskatchewan, Dept Food & Bioprod Sci, Saskatoon, SK, Canada

来源：

INSECT MOLECULAR BIOLOGY | 2010年 / 19卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

mucin; peritrophic matrix; midgut; serine protease; Mamestra configurata; FRUGIPERDA LEPIDOPTERA LARVAE; MOSQUITO ANOPHELES-GAMBIAE; DIGESTIVE ENZYMES; SPODOPTERA-FRUGIPERDA; BERTHA ARMYWORM; AEDES-AEGYPTI; HELICOVERPA-ARMIGERA; MEMBRANE-STRUCTURE; SURFACE MUCIN; CDNA CLONING;

D O I：

10.1111/j.1365-2583.2009.00966.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insect intestinal mucins (McIIM2-4) expressed in the midgut of feeding, starved and moulting Mamestra configurata larvae were identified. McIIM2 and McIIM4 were associated with the peritrophic matrix (PM). PMs from feeding and starved larvae were translucent and contained organized chitin bundles perpendicular to their long axis, whereas PM from moulting larvae consisted of an inner opaque mass surrounded by an outer translucent sleeve. Serine protease genes (McSP1, McSP2, McSP25 and McSP29) were also expressed in these larvae and several serine proteases were associated with the PM. Serine protease activity was also detected in the midgut of feeding, starved and moulting larvae.

引用

页码：163 / 175

页数：13