Tuning the mechanical and physicochemical properties of cross-linked protein films

被引:8
|
作者
Parveen, Sultana [1 ]
Chaudhury, Susmitnarayan [1 ,2 ]
Dasgupta, Swagata [1 ]
机构
[1] Indian Inst Technol Kharagpur, Dept Chem, Kharagpur 721302, W Bengal, India
[2] Natl Ctr Biol Sci, GKVK Campus,Bellary Rd, Bangalore 560065, Karnataka, India
关键词
ampicillin sodium; cataractous eye protein isolate; glutaraldehyde; protein films; WATER-VAPOR PERMEABILITY; SOY PROTEIN; TENSILE PROPERTIES; EDIBLE FILMS; FUNCTIONAL-PROPERTIES; STRUCTURAL-PROPERTIES; PHYSICAL-PROPERTIES; GELATIN FILMS; RELEASE; GLUTARALDEHYDE;
D O I
10.1002/bip.23321
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Films derived from natural sources such as proteins provide an advantage over synthetic films due to their noncytotoxicity, biodegradability, and vast functionality. A new protein source gained from the cataractous eye protein isolate (CEPI) obtained after surgery has been investigated for this purpose. Glycerol has been employed as the plasticizer and glutaraldehyde (GD) as a cross-linker. Fourier transform infrared spectroscopy was employed to characterize the films. Nanoindentation and thermogravimetric analyses reveal improved mechanical and thermal properties of the cross-linked films. The films with 20% (w/w) GD exhibited properties such as the highest modulus and low water solubility. It is possible to tune the properties based on the extent of cross-linking. All the films were completely degraded by the enzyme trypsin. The similarity of these films was checked by using the prepared films as a delivery vehicle for a model compound, ampicillin sodium. The encapsulation efficiency was found to be 74%, and in vitro release studies showed significant amounts of drug release at physiological pH. This study will help us understand how the properties of protein films can be tuned to obtain the desired physicochemical properties. These biodegradable protein films could find use in pharmaceutical industries as delivery carriers.
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页数:13
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