Protective Effect of Probiotic Potentiate with Thiopental in Intestinal Ischemia-Reperfusion Induced Lung Injury

Background and Objective: Intestinal ischemia/reperfusion leads to development of multiple organ dysfunctions including lung. The aim of present investigation was to determine the effect of thiopental alone and in combination with probiotic on I/R induced lung injured rats. Materials and Methods: Animals were treated with probiotics (1 x 10(9) CFU) and thiopental (20 mg kg(-1), i.p.) alone and in combination. Probiotics was administered with 3 days prior to the surgery and thiopental was administered 15 min after the induction of reperfusion. The ELISA method was used to determine the level of mediators of inflammation and oxidative stress in the lung tissue. Moreover Western blot, RT-PCR assay were performed by the estimation of expression of several proteins in the lung tissue homogenates. Results: Data of the investigation revealed that thiopental alone and in combination with probiotic reduced the inflammatory cytokines oxidative stress in the lung tissues compared to I/R group. Moreover expression of TLR-4, iNOS and NF-kappa B proteins were reduced in the lung tissue of thiopental alone and in combination with probiotic treated group compared to I/R group of rats. Treatment with thiopental alone and in combination with probiotic attenuated the altered histopathology of intestinal I/R induced lung injured rats. Conclusion: In conclusion, data of investigation suggested that thiopental ameliorated the lung injury by regulating iNOS/TLR-4/NF-kappa B pathway in intestinal I/R induced lung injured rats model. Moreover study also revealed that thiopental showed synergistic effect against intestinal I/R induced lung injury when treated with probiotics.