Cloning and sequencing of the Thermus aquaticus glycerol facilitator gene

被引：0
|
作者：
Huang, HS [1 ]
Ito, K [1 ]
Yoshimoto, T [1 ]
机构：
[1] Nagasaki Univ, Sch Pharmaceut Sci, Nagasaki 852, Japan
来源：
DNA SEQUENCE | 2002年 / 13卷 / 06期
关键词：
glycerol facilitator; Thermus aquaticus; gene cloning; Escherichia coli;
D O I：
10.1080/1042517021000038745
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The gene glpK , encoding glycerol kinase of Thermus aquaticus has been identified [ Biosci. Biotechnol. Biochem. , 62 (1998) 2375-2381]. In the present work, the nucleotide sequence of glpFK operon and the gene glpF encoding glycerol facilitator were determined. T. aquaticus GlpF was predicted to contain 272 amino acids with six putative transmembrane segments and two half-membrane-spanning segments that contained the motif Asn-Pro-Ala, respectively. The amino acid residues involved in the discrimination of glycerol were deduced to be Trp44, Tyr182, and Arg188.
引用
收藏
页码：387 / 390
页数：4
相关论文
共 50 条
  • [1] Cloning, sequencing, high expression, and crystallization of the thermophile Thermus aquaticus glycerol kinase
    Huang, HS
    Ito, K
    Yin, CH
    Kabashima, T
    Yoshimoto, T
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (12) : 2375 - 2381
  • [2] Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC33923
    Tsusaki, K
    Nishimoto, T
    Nakada, T
    Kubota, M
    Chaen, H
    Fukuda, S
    Sugimoto, T
    Kurimoto, M
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1997, 1334 (01): : 28 - 32
  • [3] Thermostable glycerol kinase from Thermus flavus:: cloning, sequencing, and expression of the enzyme gene
    Huang, HS
    Kabashima, T
    Ito, K
    Yin, CH
    Nishiya, Y
    Kawamura, Y
    Yoshimoto, T
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1998, 1382 (02): : 186 - 190
  • [4] Cloning and sequencing of aspartate aminotransferase from Thermus aquaticus YT1
    OFarrell, PA
    Sannia, G
    Walker, JM
    Doonan, S
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 239 (03) : 810 - 815
  • [5] THE RECA GENE FROM THERMUS-AQUATICUS - CLONING, EXPRESSION AND PURIFICATION
    ANGOV, E
    CAMERINIOTERO, RD
    [J]. JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 155 - 155
  • [6] Isocitrate dehydrogenase from Thermus aquaticus YT1: Purification of the enzyme and cloning, sequencing, and expression of the gene
    Miyazaki, K
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (12) : 4627 - 4631
  • [7] Preliminary crystallographic study of Thermus aquaticus glycerol kinase
    Huang, HS
    Inoue, T
    Ito, K
    Yoshimoto, T
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2001, 57 : 1030 - 1031
  • [8] THE RECA GENE FROM THERMUS-AQUATICUS - CLONING, EXPRESSION AND BIOCHEMICAL-CHARACTERIZATION
    ANGOV, E
    CAMERINIOTERO, RD
    [J]. FASEB JOURNAL, 1994, 8 (07): : A1331 - A1331
  • [9] Thermus aquaticus ATCC 33923 amylomaltase gene cloning and expression and enzyme characterization:: Production of cycloamylose
    Terada, Y
    Fujii, K
    Takaha, T
    Okada, S
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (03) : 910 - 915
  • [10] Cloning and Expression of Thermus aquaticus DNA polymerase in Escherichia coli
    Roayaei, Mohammad
    Galehdari, Hamid
    [J]. JUNDISHAPUR JOURNAL OF MICROBIOLOGY, 2008, 1 (01) : 1 - 5
12345