Field evaluation of an equine influenza ELISA used in New South Wales during the 2007 Australian outbreak response

被引：13

作者：

Sergeant, Evan S. G. ^{[1
]}

Kirkland, Peter D. ^{[2
]}

Cowled, Brendan D. ^{[3
]}

机构：

[1] New S Wales Dept Primary Ind, Orange, NSW 2800, Australia

[2] Elizabeth Macarthur Agr Inst, New S Wales Dept Primary Ind, Camden, NSW 2570, Australia

[3] Off Chief Vet Officer, Dept Agr Fisheries & Forestry, Canberra, ACT 2601, Australia

来源：

PREVENTIVE VETERINARY MEDICINE | 2009年 / 92卷 / 04期

关键词：

Equine influenza; bELISA; Receiver operating characteristic; ROC;

D O I：

10.1016/j.prevetmed.2009.08.025

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

During the Australian epidemic of equine influenza in 2007, tens of thousands of horses were infected. From the resulting field data, 475 known infected and 1323 uninfected horses were identified to allow a post outbreak evaluation of the performance of the commonly used bELISA for influenza A under field conditions. A variety of techniques, such as ROC plots, area under the curve and hypothesis testing were used to assess the overall performance of the test. The test was deemed to be accurate (area under curve = 0.993 +/- 0.003 standard error) and significantly informative (z=-32.0; p<0.0001). Sensitivity and specificity of the test as used in the response (cut-point percentage inhibition >= 50) were 0.992 (95% CI: 0.979-0.997) and 0.967 (95% CI: 0.957-0.976), respectively. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：382 / 385

页数：4

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