Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry

被引:29
|
作者
Kim, Hyung Min [1 ]
Park, Ju-Hwan [1 ]
Nguyen Phuoc Long [1 ]
Kim, Dae-Duk [1 ,2 ]
Kwon, Sung Won [1 ,2 ]
机构
[1] Seoul Natl Univ, Coll Pharm, Seoul 08826, South Korea
[2] Seoul Natl Univ, Res Inst Pharmaceut Sci, Seoul 08826, South Korea
基金
新加坡国家研究基金会;
关键词
Cardiovascular drugs; Dried blood spot; LC-MS/MS; Therapeutic drug monitoring; LC-MS/MS METHOD; VALIDATION; SAMPLES; ASSAY; QUANTIFICATION; LUMEFANTRINE; HEMATOCRIT; HRMS;
D O I
10.1016/j.jfda.2019.06.001
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
A dried blood spot (DBS) sampling method was exploited to extract cardiovascular drugs using a small volume of whole blood of human and rodent. Thereafter, an analytical method using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of 12 cardiovascular drugs. A 6 mm internal diameter disc containing 10 mu L of blood was punched from a specifically designed card and analyzed by LC-MS/MS using a gradient elution method with a total run time of 16 min. For sample separation, a universal octadecyl-silica column was used with a flow rate of 0.2 mL/min. The developed method was validated in terms of linearity, accuracy, and precision, which showed satisfactory results. In addition, the matrix effects were closely investigated to confirm the extraction efficiency. Additionally, the stability was tested by storing DBSs at room temperature; the results showed that these drugs were stable for at least 30 days. Accordingly, the proposed LC-MS/MS method is capable to analyze several cardiovascular drugs in a single analysis. It can be applied to therapeutic drug monitoring in patients as well as in the in vivo settings. Copyright (C) 2019, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.
引用
收藏
页码:906 / 914
页数:9
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