Optimization of Parameters for Electrochemical Detection: Computer Simulation and Experimental Study

It has been shown that for each electrochemical measurement it is necessary first to optimize all the parameters that influence the obtained electrochemical signal. This is a time-consuming process and that is why in this study we focused on the computer simulation of the influence of multiparameters on the electrochemical signal. Herein we used a model system of the electroreduction of ruthenium(III) salt on gold electrode in phosphate buffer solution. Ru3+ is often used as redox probe for detection and understanding of nucleic acid hybridization on the electrodes. This study should reveal some new aspects of using the computer simulations in determining the optimal measuring conditions for detection of RNA/PNA hybridoms on the gold surface.