A comparison of different modes for the detection of p53 protein accumulation - A study of bladder cancer

被引：10
|
作者：
Kraggerud, SM
Jacobsen, KD
Berner, A
Stokke, T
Holm, R
Smedshammer, L
BorresenDale, AL
Fossa, SD
机构：
[1] NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT CELL BIOL,N-0310 OSLO,NORWAY
[2] NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT PATHOL,N-0310 OSLO,NORWAY
[3] NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT BIOPHYS,N-0310 OSLO,NORWAY
[4] NORWEGIAN RADIUM HOSP,INST CANC RES,DEPT MED ONCOL & RADIOTHERAPY,N-0310 OSLO,NORWAY
来源：
PATHOLOGY RESEARCH AND PRACTICE | 1997年 / 193卷 / 07期
关键词：
bladder cancer; p53; protein; immunoblotting; immunohistochemistry; flow cytometry;
D O I：
10.1016/S0344-0338(97)80099-4
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
The aim of the present study was to evaluate different techniques for the analysis of p53 protein accumulation in human bladder cancer. The accumulation was evaluated in 23 carcinomas by immunoblotting (IB), immunohistochemistry (IHC) and flow cytometry (FCM). The results revealed that six (26%), eight (35%) and ten (43%) of the tumours were p53 protein positive by LB, IHC and FCM, respectively. Mutation analysis of the TP53 gene confirmed mutations in 8 of 9 tumours which showed increased levels of p53 protein by FCM. Our results indicate that IHC could be applied for studies of p53 protein accumulation in archival formalin fixed, paraffin-embedded bladder tumours. However, FCM is a more sensitive and objective method for the detection of p53 protein than IHC and this should be taken into account when routinely evaluating the p53 protein accumulation by IHC.
引用
收藏
页码：471 / 478
页数：8
相关论文
共 50 条
  • [1] Clinical significance of nuclear p53 protein accumulation in bladder cancer
    Toktaş G.
    Türkeri L.N.
    Ünluer E.
    Çalişkan M.
    Aksoy B.
    Akdaş A.
    [J]. International Urology and Nephrology, 1999, 31 (3) : 327 - 334
  • [2] Comparison of P53 protein overexpression with P53 mutation in bladder cancer: Clinical and biologic aspects
    Vet, JAM
    Bringuier, PP
    Schaafsma, HE
    Witjes, JA
    Debruyne, FMJ
    Schalken, JA
    [J]. LABORATORY INVESTIGATION, 1995, 73 (06) : 837 - 843
  • [3] Analysis of p53 status in bladder cancer: Mutation, LOH and protein accumulation
    不详
    [J]. EUROPEAN CYTOKINE NETWORK, 2004, 15 (04) : XI - XI
  • [4] Comparison of Nuclear Accumulation of p53 Protein with Mutations in the p53 Gene of Human Breast Cancer Tissues
    范萍
    武正炎
    查小明
    王萱仪
    [J]. The Journal of Biomedical Research, 2001, (02) : 91 - 95
  • [5] Immunohistochemical detection of p53 protein accumulation in head and neck cancer:: Correlation with p53 gene alterations
    Taylor, D
    Koch, WM
    Zahurak, M
    Shah, K
    Sidransky, D
    Westra, WH
    [J]. HUMAN PATHOLOGY, 1999, 30 (10) : 1221 - 1225
  • [6] Manipulation of p53 Protein in Bladder Cancer Treatment
    Mansor, Siti Farizan
    [J]. IIUM MEDICAL JOURNAL MALAYSIA, 2021, 20 (01): : 137 - 147
  • [7] P53 NUCLEAR-PROTEIN ACCUMULATION CORRELATES WITH MUTATIONS IN THE P53 GENE, TUMOR GRADE, AND STAGE IN BLADDER-CANCER
    ESRIG, D
    SPRUCK, CH
    NICHOLS, PW
    CHAIWUN, B
    STEVEN, K
    GROSHEN, S
    CHEN, SC
    SKINNER, DG
    JONES, PA
    COTE, RJ
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 1993, 143 (05): : 1389 - 1397
  • [8] Study of p53 and heat shock protein (HSP) manifestations in bladder cancer
    Jang, Seok Heun
    Kim, Jae Il
    Son, Jeong Hwan
    [J]. INTERNATIONAL JOURNAL OF UROLOGY, 2010, 17 : A302 - A303
  • [9] P53 accumulation, deoxyribonucleic acid ploidy and progression of bladder cancer
    Raitanen, MP
    Tammela, TLJ
    Kallioinen, M
    Isola, J
    [J]. JOURNAL OF UROLOGY, 1997, 157 (04): : 1250 - 1253
  • [10] ACCUMULATION OF NUCLEAR P53 AND TUMOR PROGRESSION IN BLADDER-CANCER
    ESRIG, D
    ELMAJIAN, D
    GROSHEN, S
    FREEMAN, JA
    STEIN, JP
    CHEN, SC
    NICHOLS, PW
    SKINNER, DG
    JONES, PA
    COTE, RJ
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1994, 331 (19): : 1259 - 1264
12345