Molecular design and bio-activity analysis of a t-PA derivative (t-PA S165W)

In order to obtain tissue-type plasminogen activator (t-PA) mutant with enhanced affinity for fibrin, redesigning t-PA strategies were proposed by computer molecular designing technology, and the re-designed t-PA derivative, t-PA S165W, was expressed in CHO cells. The bio-activities and affinity for fibrin of the expression product were tested. No apparent changes were observed between t-PA S165W and wild type t-PA. Therefore, single site mutation of the t-PA could not lead to enhanced affinity for fibrin of t-PA.