Widespread activation of the DNA damage response in human pancreatic intraepithelial neoplasia

被引:30
|
作者
Koorstra, Jan-Bart M. [1 ,2 ,4 ]
Hong, Seung-Mo [1 ,4 ]
Shi, Chanjuan [1 ]
Meeker, Alan K. [1 ]
Ryu, Ji Kon [1 ]
Offerhaus, George Johan A. [2 ]
Goggins, Michael G. [1 ,3 ]
Hruban, Ralph H. [1 ,3 ]
Maitra, Anirban [1 ,3 ,4 ]
机构
[1] Johns Hopkins Univ, Sol Goldman Pancreat Canc Res Ctr, Sch Med, Dept Pathol, Baltimore, MD 21231 USA
[2] Univ Med Ctr, Dept Pathol, Utrecht, Netherlands
[3] Johns Hopkins Univ, Sol Goldman Pancreat Canc Res Ctr, Sch Med, Dept Oncol, Baltimore, MD 21231 USA
[4] Johns Hopkins Univ, McKusick Nathans Inst Genet Med, Sch Med, Baltimore, MD 21231 USA
关键词
pancreatic cancer; pancreatic intraepithelial neoplasia; DNA damage; ATM; Chk2; ONCOGENE-INDUCED SENESCENCE; DUCTAL ADENOCARCINOMA; CELLULAR SENESCENCE; TISSUE MICROARRAY; CANCER; CELLS; EXPRESSION; LESIONS; PROGRESSION; PHOSPHORYLATION;
D O I
10.1038/modpathol.2009.114
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Pancreatic intraepithelial neoplasia (PanIN) lesions are the most common non-invasive precursors of pancreatic adenocarcinoma. We postulated that accumulating DNA damage within the PanIN epithelium activates checkpoint mechanisms. Tissue microarrays were constructed from 81 surgically resected primary pancreatic adenocarcinomas and an independent set of 58 PanIN lesions (31 PanIN-1, 14 PanIN-2, and 13 PanIN-3). Immunohistochemical labeling was carried out using anti-gamma H2AX(Ser139), anti-phosphoATM(Ser1981), anti-phosphoChk2(Thr68), and anti-p53. A 'histologic score' combining area and intensity of labeling in the nuclear compartment was determined for each lesion. A progressive increase in gamma H2AX(Ser139) labeling, consistent with escalating DNA damage, was observed in the non-invasive precursor lesions (scores of 4.34, 6.21, and 7.50, respectively, for PanIN-1, -2, and -3), compared with the pancreatic ductal epithelium (score 2.36) (ANOVA, P<0.0001). In conjunction, activation of the ataxia telangiectasia mutated (ATM)-Chk2 checkpoint pathway was observed in all histological grades of PanIN lesions. Specifically, pATM(Ser1981) histologic scores for PanIN-1, PanIN-2, and PanIN-3 were 4.83, 5.14, and 7.17, respectively, versus 2.33 for the ductal epithelium (ANOVA, P<0.0001); the corresponding scores for pChk2(Thr68) were 5.43, 7.64, and 5.44 in PanINs-1, -2, and -3, respectively, versus 2.75 in the ductal epithelium (ANOVA, P<0.0001). In contrast, absent to minimal nuclear p53 was observed in the ductal epithelium, and in PanINs-1 and -2 (a histologic score of 0-1.86), with a significant upregulation (corresponding to mutational inactivation) seen only at the stage of PanIN-3 and invasive neoplasia (histologic scores of 4.00 and 4.22). Nuclear p53 accumulation in cancers was associated with attenuation of the ATM-Chk2 checkpoint and a restitution to 'baseline' levels. To conclude, activation of the ATM-Chk2 checkpoint pathway is commonly observed in PanINs, likely in response to the accumulating DNA damage from events such as oncogene mutations and telomere dysfunction. Loss of p53 function appears to be a critical determinant for bypassing this checkpoint and the subsequent progression to invasive adenocarcinoma. Modern Pathology (2009) 22, 1439-1445; doi: 10.1038/modpathol.2009.114; published online 7 August 2009
引用
收藏
页码:1439 / 1445
页数:7
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