Taking Anhui province as an example, regional sustainable development has been studied and analyzed. The index system includes twenty-seven indexes, which have been chosen from economy, society and environment. Principal component analysis method and comprehensive evaluation method have been applied in the evaluation of sustainable development level of Anhui province. According to results, Anhui province has been divided into four function subareas. Through the analysis we can conclude that it is more comprehensive and scientifically by two methods. The research can provide science reference for the study of evaluation of regional sustainable development.