Reduction of viral load in whitefly (Bemisia tabaci Gen.) feeding on RNAi-mediated bean golden mosaic virus resistant transgenic bean plants

被引:7
|
作者
de Paula, Nayhanne T. [1 ,2 ]
de Faria, Josias C. [3 ]
Aragao, Francisco J. L. [1 ]
机构
[1] Embrapa Recursos Genet & Biotecnol, BR-70770900 Brasilia, DF, Brazil
[2] Univ Brasilia, Dept Biol Celular, BR-70910900 Brasilia, DF, Brazil
[3] Embrapa Arroz & Feijao, BR-75375000 Santo Antonio De Goias, Go, Brazil
关键词
Bean golden mosaic virus; GM plants; Viral load; Virus resistance; Whitefly; PHASEOLUS-VULGARIS; GEMINIVIRUSES; BEGOMOVIRUSES; ALEYRODIDAE;
D O I
10.1016/j.virusres.2015.08.012
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The RNAi concept was explored to silence the rep gene from the bean golden mosaic virus (BGMV) and a genetically modified (GM) bean immune to the virus was previously generated. We investigated if BGMV-viruliferous whiteflies would reduce viral amount after feeding on GM plants. BGMV DNA amount was significantly reduced in whiteflies feeding in GM-plants (compared with insects feeding on non-GM plants) for a period of 4 and 8 days in 52% and 84% respectively. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:245 / 247
页数:3
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