Association of an isolate of papaya leaf curl virus and papaya leaf curl betasatellite with leaf curl disease of radish in India

Radish (Raphanus sativus L.) plants of the cultivar Pusa Reshmi exhibiting severe leaf curl symptoms were observed in the cultivated fields at Lucknow with significant disease incidence. The virus was whitefly transmittable and induced leaf curl symptoms by 24 days post inoculation. PCR with two sets of degenerate primers confirmed the presence of a begomovirus and its associated betasatellite. Further, the full-length viral genome was amplified by RCA and two clones of similar to 2.7 kb were sequenced (KY026598 and KY026597) which shared the highest (95.25%) sequence identity and close phylogenetic relationships with papaya leaf curl virus (PaLCuV), therefore, identified as isolates of PaLCuV. The associated betasatellite was identified as papaya leaf curl betasatellite (PaLCuB) based on its highest sequence identity (98.32%) and close relationship with PaLCuB.