Development and characterization of microsatellite markers for giant sequoia, Sequoiadendron giganteum (Cupressaceae)

被引：4
|
作者：
DeSilva, Rainbow [1 ]
Dodd, Richard [1 ]
机构：
[1] Univ Calif Berkeley, Berkeley, CA 94720 USA
来源：
CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 01期
关键词：
Microsatellite Marker; Significant Linkage Disequilibrium; Short Sequence Repeat; LIZ500 Size Standard; Endemic Flora;
D O I：
10.1007/s12686-013-0039-x
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
We describe eleven polymorphic microsatellite markers for giant sequoia [Sequoiadendron giganteum (Lindl.) J. Buchh.], the world's largest tree. Size-selected genomic DNA was enriched for short sequence repeats (SSR) and the resulting SSR library was analyzed on a Roche 454 platform. DNA samples from seedlings grown in a common garden (n = 359), representing a gene pool from twenty-three groves, were examined for SSR polymorphisms. The total observed alleles per locus ranged from three to twenty-five. Samples from the cabin creek grove (n = 21) exhibit observed heterozygosities ranging from 0.19 to 1.0, with one loci displaying significant deviation from Hardy-Weinberg equilibrium. Within this grove, no loci showed significant linkage disequilibrium. These microsatellite markers are important for assessing and conserving genetic diversity in this rare conifer.
引用
收藏
页码：173 / 174
页数：2
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