Paraburkholderia flava sp. nov., isolated from cool temperate forest soil

A Gram-stain-negative, aerobic and short rod-shaped bacterial strain, designated LD6(T), was isolated from a forest soil sample in Suwon, Gyeonggi-do, Republic of Korea. Strain LD6(T) grew at 10-37 degrees C (optimal temperature. 28 degrees C). and tolerated pH 8.0 and 2% (w/v) NaCl. Strain LD6(T) was related most closely to members of the genus Paraburkholderia, namely Paraburkholderia azotifigens NF2-5-3(T) (98.2% 165 rRNA gene sequence similarity), P. megapolitana A3(T) (97.9%), P. ginsengiterrae DCY85(T) (97.9%) and P. caribensis MWAP64(T) (97.7%). The strain grew well on R2A agar, tryptone soya agar, Mueller-Hinton agar and nutrient agar. The major polar lipid profile comprised phosphatidylglycerol. diphosphatidylglycerol, phosphatidylethanolamine, aminophospho- lipid and glycolipid. The major respiratory quinone was ubiquinone 8 (Q-8). The main fatty acids were C-17:0 cyclo, C-16:0, C-16:0 3-OH, C-19(.0) cyclo omega 8c and C-12:0. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.4 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain LD6(T) and its reference type strains ranged from 80.3 to 82.4%, and from 23.7 to 33.7%. respectively. Based on phenotypic, chemotypic and genotypic evidence, strain LD6(T) could be differentiated phylogenetically and phenotypically from the recognized species of the genus Paraburkholderia. Therefore, strain LD6(T) is considered to represent a novel species, for which the name Paraburkholderia flava sp. nov. is proposed. The type strain is LD6(T) (=KACC 21387(T)=JCM 33640(T)).