Coherent diffractive imaging of microtubules using an X-ray laser

被引：21

作者：

Branden, Gisela ^{[1
]}

Hammarin, Greger ^{[1
]}

Harimoorthy, Rajiv ^{[1
]}

Johansson, Alexander ^{[1
]}

Arnlund, David ^{[1
]}

Malmerberg, Erik ^{[2
]}

Barty, Anton ^{[3
]}

Tangefjord, Stefan ^{[4
]}

Berntsen, Peter ^{[1
]}

DePonte, Daniel P. ^{[5
]}

Seuring, Carolin ^{[3
,6
]}

White, Thomas A. ^{[3
]}

Stellato, Francesco ^{[3
]}

Bean, Richard ^{[3
]}

Beyerlein, Kenneth R. ^{[3
]}

Chavas, Leonard M. G. ^{[3
]}

Fleckenstein, Holger ^{[3
]}

Gati, Cornelius ^{[3
]}

Ghoshdastider, Umesh ^{[7
]}

Gumprecht, Lars ^{[3
]}

Oberthuer, Dominik ^{[3
]}

Popp, David ^{[7
]}

Seibert, Marvin ^{[5
]}

Tilp, Thomas ^{[3
]}

Messerschmidt, Marc ^{[5
]}

Williams, Garth J. ^{[5
]}

Loh, N. Duane ^{[8
]}

Chapman, Henry N. ^{[3
,6
,9
]}

Zwart, Peter ^{[2
]}

Liang, Mengning ^{[3
,5
]}

Boutet, Sebastien ^{[5
]}

Robinson, Robert C. ^{[7
,10
,11
]}

Neutze, Richard ^{[1
]}

机构：

[1] Univ Gothenburg, Dept Chem & Mol Biol, Box 462, S-40530 Gothenburg, Sweden

[2] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, 1 Cyclotron Rd, Berkeley, CA 94720 USA

[3] DESY, Ctr Free Electron Laser Sci, D-22607 Hamburg, Germany

[4] AstraZeneca R&D, Discovery Biol, Pepparredsleden 3, S-43189 Molndal, Sweden

[5] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA USA

[6] Hamburg Ctr Ultrafast Imaging, D-22761 Hamburg, Germany

[7] ASTAR, Inst Mol & Cell Biol, Biopolis, Singapore 138673, Singapore

[8] Natl Univ Singapore, Dept Phys, Singapore 117551, Singapore

[9] Univ Hamburg, Dept Phys, D-22761 Hamburg, Germany

[10] Natl Univ Singapore, Dept Biochem, Singapore 117597, Singapore

[11] Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

瑞典研究理事会; 美国国家卫生研究院;

关键词：

SLOWLY HYDROLYZABLE ANALOG; CRYO-EM; DYNAMIC INSTABILITY; STRUCTURAL-CHANGES; GTP HYDROLYSIS; TUBULIN; ANGSTROM; BINDING; RECONSTRUCTION; INFORMATION;

D O I：

10.1038/s41467-019-10448-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature.

引用

页数：9

共 50 条

[1] Coherent diffractive imaging of microtubules using an X-ray laser
Gisela Brändén
Greger Hammarin
Rajiv Harimoorthy
Alexander Johansson
David Arnlund
Erik Malmerberg
Anton Barty
Stefan Tångefjord
Peter Berntsen
Daniel P. DePonte
Carolin Seuring
Thomas A. White
Francesco Stellato
Richard Bean
Kenneth R. Beyerlein
Leonard M. G. Chavas
Holger Fleckenstein
Cornelius Gati
Umesh Ghoshdastider
Lars Gumprecht
Dominik Oberthür
David Popp
Marvin Seibert
Thomas Tilp
Marc Messerschmidt
Garth J. Williams
N. Duane Loh
Henry N. Chapman
Peter Zwart
Mengning Liang
Sébastien Boutet
Robert C. Robinson
Richard Neutze
[J]. Nature Communications, 10
[2] Author Correction: Coherent diffractive imaging of microtubules using an X-ray laser
Gisela Brändén
Greger Hammarin
Rajiv Harimoorthy
Alexander Johansson
David Arnlund
Erik Malmerberg
Anton Barty
Stefan Tångefjord
Peter Berntsen
Daniel P. DePonte
Carolin Seuring
Thomas A. White
Francesco Stellato
Richard Bean
Kenneth R. Beyerlein
Leonard M. G. Chavas
Holger Fleckenstein
Cornelius Gati
Umesh Ghoshdastider
Lars Gumprecht
Dominik Oberthür
David Popp
Marvin Seibert
Thomas Tilp
Marc Messerschmidt
Garth J. Williams
N. Duane Loh
Henry N. Chapman
Peter Zwart
Mengning Liang
Sébastien Boutet
Robert C. Robinson
Richard Neutze
[J]. Nature Communications, 10
[3] Coherent diffractive imaging of microtubules using an X-ray laser (vol 10, 2589, 2019)
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[J]. INTERNATIONAL CONFERENCE ON ADVANCED PHASE MEASUREMENT METHODS IN OPTICS AN IMAGING, 2010, 1236 : 200 - 200
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[10] Beyond crystallography: Diffractive imaging using coherent x-ray light sources
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