Rapid detection of Staphylococcus aureus bacteremia and methicillin resistance by real-time PCR in whole blood samples

被引：21

作者：

Wellinghausen, N. ^{[1
,2
]}

Siegel, D. ^{[2
]}

Gebert, S. ^{[2
]}

Winter, J. ^{[2
]}

机构：

[1] Dr Gaertner & Partner Labs, D-88212 Ravensburg, Germany

[2] Univ Hosp Ulm, Inst Med Microbiol & Hyg, Ulm, Germany

来源：

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2009年 / 28卷 / 08期

关键词：

POLYMERASE-CHAIN-REACTION; STREAM INFECTIONS; PATHOGENS; DNA;

D O I：

10.1007/s10096-009-0723-7

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We prospectively evaluated a real-time polymerase chain reaction (PCR) approach for the rapid diagnosis of Staphylococcus aureus bacteremia and presence of the mecA gene in 902 blood samples from 468 infectious episodes of 384 patients. Eight of 12 blood culture (BC)-confirmed samples were positive by the S. aureus-specific PCR. In addition, the mecA gene PCR correctly detected all cases of BC-confirmed methicillin-resistant Staphylococcus aureus (MRSA) infection. A positive PCR result was also obtained in ten of 462 BC-negative infectious episodes, including three patients with culture-confirmed S. aureus infection at other body sites.

引用

页码：1001 / 1005

页数：5

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